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【答案】应助回帖
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80后的末班车: 金币+30, 翻译EPI+1, ★★★★★最佳答案, 非常感谢 2014-12-01 20:05:50
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A precise quantity (~15 mg) of polysaccharide sample was weighed and put in a 10 mL test tube with a plug, to which 1 mL distilled water was added. The test tube was heated such that the polysaccharide sample was dissolved, and 2 mL trifluoroacetic acid (2 mol•L-1) was added. The mixed solution was shaken uniformly and sealed. The test tube was then placed in an oven, where it was heated at 110 ℃ for hydrolysis. After 8 h it was taken out, cooled to ambient temperature, and centrifuged at 12000 r•min-1 for 5 min. The supernatant was collected and transferred to a 10 mL volumetric flask, where it was neutralized by NaOH (2 mol•L-1) to pH = ~7.0, diluted with water to volume, and shaken uniformly for PMP derivation and content measurement. The chromatographic column is a PLATISIL ODS column (250 mm × 4.6 mm, 5 μm) made by Dikma Technologies Inc. The column temperature is 35℃. The mobile phase is acetonitrile - 20 mmol•L-1 ammonium acetate buffer（22∶78） with pH = 5.5. The flow rate is 1.0 mL•min-1. The UV detection wavelength is 245 nm. The injection volume is 10 μL. |
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