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huojinlong8610金虫 (小有名气)
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[求助]
润色英文摘要
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摘 要：白细胞介素6（IL-6）是机体重要的免疫调节因子，在佐剂的应用方面具有很好的发展前景。本研究以版纳微型猪近交系（BMI）为实验动物，通过RT-PCR法获得IL-6基因编码区序列，提交GenBank数据库，登录号为“JQ839263”。对BMI IL-6基因和相应的蛋白质序列进行了多重生物信息学分析，结果显示该基因编码212个氨基酸，蛋白分子量（Mw）为23.88 kD，等电点（pI）为6.66，存在一个保守结构域，一个跨膜结构，N端和C端均疏水，且在N端存在信号肽，有89%的可能性定位于细胞核。将BMI的IL-6基因编码区序列与NCBI下载到的4条猪IL-6基因序列进行比对结果显示BMI IL6与NM_214399和DQ832259核酸序列完全相同，与AF309651和AF518322各存在1处碱基差异；多物种氨基酸序列比对及系统进化分析表明，BMI与骆驼亲缘关系最近。同时利用半定量PCR法确定了IL-6基因mRNA在BMI 12个组织中的表达量，发现在肺、皮肤、肌肉中表达量较高。本研究为进一步研究猪IL-6基因的表达调控奠定了理论基础。 Abstract：The interleukin-6 (IL-6) is an important immune regulatory factor, and has great development prospects in the application of adjuvants. Using the Banna Mini-pig Inbred Line (BMI) as the experimental animal, we successfully cloned the coding region of porcine IL-6 gene through the reverse transcriptase polymerase chain reaction (RT-PCR) method, and had deposited the sequence into NCBI database and assigned to the accession No.: JQ839263. Then we further study this gene by multiple bioinformatics analyses method. Results showed that BMI IL-6 encoded 212 amino acids with a predicted molecular weight of 23.88 kD and the isoelectric point (pI) 6.66 and a signal peptide in N-terminal. It is a nuclear protein with a 89% probability. In addition, BMI IL-6 contained one conserved domain and its N-terminal and C-terminal were both hydrophobic. Comparing the sequence of the coding region of BMI IL-6 gene with the download 4 pig IL-6 gene sequences, the result shows BMI is identical with NM_214399 and DQ832259, only had 1 base pair difference with AF309651 and AF518322 respectively. Multi-species amino acid sequence alignment and phylogenetic analysis showed that BMI has a closest relationship with camel. Finally, we analysed IL-6 gene mRNA expression in 12 important tissues of BMI through RT-PCR method, results showed that BMI IL-6 gene was highly expressed in lung, skin and muscle of BMI adult pig. These data serve as a foundation for further insight into the expression and regulation of this porcine gene. |
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至尊木虫 (知名作家)
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RXMCDM: 金币+2, 多谢应助！ 2014-12-01 08:23:32
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中文部分也需要润色： 1、通过RT-PCR法获得IL-6基因编码区序列：通过RT-PCR法获得IL-6 mRNA编码区序列（不能用基因代替mRNA，反之亦然）； 2、存在一个保守结构域：这个描述缺乏具体性，应该具体指出该结构域的名字; 3、多物种氨基酸序列比对及系统进化分析表明，BMI与骆驼亲缘关系最近：多物种氨基酸序列比对及系统进化分析表明，就IL-6而言BMI与骆驼亲缘关系最近（读你的原话会误以为BMI和骆驼亲缘关系最近！） 4、同时利用半定量PCR法确定了IL-6基因mRNA：同时利用半定量RT-PCR法确定了IL-6基因mRNA（RT-PCR不能与PCR混称）。 5、提交GenBank数据库，登录号为“JQ839263”：此内容无需写于摘要中（可放脚注） 另外：”有89%的可能性定位于细胞核“，此结论在文中有什么意义？众所周知IL-6是一种分泌蛋白（所以有信号肽），IL-6难道也定位于细胞核吗（我未去确证，但感觉即便有也不是主要的）。 英文部分： Abstract：Interleukin-6 (IL-6) 【无需用The】 is an important immune regulatory factor, and has great development prospects in the application of adjuvants. Using 【此处的the也要去掉】 Banna Mini-pig Inbred Line (BMI) as the experimental animalS【此处最好用复数】, we successfully cloned the coding region of porcine IL-6 mRNA 【用mRNA而非gene，你并非克隆了基因】 through the 【此the可去掉】 reverse transcriptase polymerase chain reaction (RT-PCR) method【无需用method】, and had 【摘要中不需要用过去完成时，去掉had】 deposited the sequence into NCBI database and assigned to the accession No.: JQ839263. Then we further study 【时态统一，用过去时studied】this gene by multiple bioinformatics analyses method【不需要用method，多余】. Results showed that BMI IL-6 encoded 【这里该用一般现在时encode，描述结论时用现在时】 212 amino acids with a predicted molecular weight of 23.88 kD and【将and换为逗号，】 the isoelectric point (pI) 6.66 and a signal peptide in【at】 N-terminal【terminus】. It is a nuclear protein with a 89% probability【建议省去次句话，所以就不改英文说法了】. In addition, BMI IL-6 contains【用现在时】 one conserved domain and both its N- and C-termini 【此处修改了，注意对照】are 【现在时】 hydrophobic. Comparing the sequence of the coding region of BMI IL-6 mRNA 【仍然不能用gene替代mRNA】 with the download 【该用downloaded，其实该词多余】 four 【不用阿拉伯数字】 pig IL-6 gene【该用mRNA】 sequences, the【去掉此the】 result shows 【加上：that the sequence of】 BMI is identical with NM_214399 and DQ832259, only has【用现在时】 one【不用阿拉伯数字】 base pair difference with AF309651 and AF518322 respectively. Multi-species amino acid sequence alignment and phylogenetic analysis showed that 【加上：IL-6 of】BMI 【最好加上origin】has a【改用the】 closest relationship with 【加上that of】 camel. Finally, we analysed IL-6 gene【此gene去掉】 mRNA expression in 12 important tissues of BMI through 【加上semi-quantitative】 RT-PCR method【此处的method可去掉】, results showed that BMI IL-6 gene 【用mRNA替代gene】 was highly expressed in lung, skin and muscle of BMI adult pig. These data lay the foundation【此处做了修改】 for further studies of the expression and regulation of this porcine gene. 草草修改了一下，仅供参考 |
2楼2014-11-30 22:05:52
mac194
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这不是摘要,所有重点句的总和不一定就是摘要 除非实验步骤新颖,不需赘述 请教: 你的实验结果和以下 2010 文章有何关联,比人家强多少 ??? https://www.biopharminternationa ... ticle/detail/651187 -----  2010 相关文章.jpg |
3楼2014-12-01 11:26:20