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3.3. SEM micrographs of PLGA/PHBV scaffold The morphology of the pure PLGA scaffold and PLGA/PHBV scaffolds containing different amount of PHBV microspheres were shown in Fig. 3. The SEM graphs (A, B, C and D) showed the bonding between PHBV microspheres and PLGA matrix. The PHBV microspheres were distributed all across the PLGA matrix and most of them were buried in the PLGA matrix which might make them hard to separate from the matrix. Graphs A, C and E demonstrated that all of the three kinds of scaffold possessed interconnected porous structure. It can be observed from graphs A and C that the addition of PHBV microspheres made the morphology of the PLGA/PHBV scaffold much rougher than that of pure PLGA scaffold. However, with the increase of PHBV microspheres, the pore size of PLGA/PHBV scaffolds changed little. The PLGA matrix walls were decorated with PHBV microspheres which can be seen from graphs B and D and they were seldom influenced by PHBV microspheres, which can be convinced by the well-connected walls. 3.4. Porosity of PLGA/PHBV scaffold Some parameters related to the scaffolds such as porosity,average volume, pore volume per gram and density were exhibited in Fig. 4. The porosities of three kinds of scaffold were slightly below the pre-set value. The porosity of pure PLGA scaffolds reached to 81.830±1.723%, which was close to that of PLGA/30% PHBV scaffolds (81.273±2.192%). When the PHBV microspheres content came to 50%, the porosity of the scaffolds still showed no statistical significant when compared with that of pure PLGA scaffolds. The result demonstrated that the PHBV microspheres had little influence on the porosity of PLGA scaffold. With the increase of PHBV microspheres, the density of scaffolds increased accordingly (from 0.17290±0.00461 of pure PLGA to 0.25160±0.03828 of PLGA/50% PHBV). At the same time, the pore volume per gram decreased with the increase of PHBV microspheres. The above two graphs coincided well with each other. Because of the augment of density, the volume of every gram of the scaffolds should have reduced. |
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