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2.4. Microsphere size analysis A light-scattering particle size analyzer (Matersizer 2000, Malvern InstrumentLtd., British) was used to determine the size distribution of the PHBV microspheres.The desiccated microspheres were suspended in a large amount of distilled water (about 800 ml) and analyzed after continuous stirring. 2.5. Morphological characterization Morphological characterization was conducted using scanning electron microscopy (SEM, FEI Quanta 200, Netherlands). PHBV microspheres and the crosssection of PLGA/PHBV scaffolds were fixed on a cupreous stub and sparked with gold. PHBV microspheres were sprinkled over conductive adhesive and their morphology was characterized by SEM using an accelerating voltage of 15 kV. PLGA/PHBV scaffolds were coated two times before observation. 2.6. Pore parameters of PLGA/PHBV scaffold The porosity and other pore parameters of the scaffold were determined according to the method described in the reference [33]. Firstly, certain amount of ethanol was added into the weighing bottle and then a scaffold (dry weight,Ws) was put into it until utterly wetting. Subsequently, weighed the bottle together with the scaffold and marked it as Wa. Record the mass of the remaining liquid together with bottle after getting out the scaffold asWb. The weight of a pycnometer which was full of ethanol was recorded as W1. The wet scaffold was transferred to the pycnometer quickly, then marked their total weight as W2. The pore parameters of the scaffolds were calculated by the equations listed below: |
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