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Polymer scaffolds, particularly in the form of microspheres, have been employed to support cells growth and deliver drugs or growth factors in tissue engineering. In this study, we have established a scaffold by embedding poly (β-hydroxybutyrate-co-β-hydroxyvalerate) (PHBV) microspheres into poly (L-lactic-coglycolic acid) (PLGA）matrix, according to their different solubility in acetone, with the aim of repairing bone defects. PLGA/PHBV scaffolds had good pore parameters, for example, the porosity of PLGA/30% PHBV scaffold can reach to 81.273 ± 2.192%. Besides, the pore size distribution of the model was evaluated and the results revealed that the pore size mainly distributed between 50 μm and 200 μm. With increasing the amount of PHBV microspheres, the compressive strength of the PLGA/PHBV scaffold enhanced. The morphology of the hybrid scaffold was rougher than that of pure PLGA scaffold, which had no significant effect on the cell behavior. The in vitro evaluation suggested that the model is suitable as a scaffold for engineering bone tissue, and has the potential for further applications in drug delivery system. |
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| 高分子支架，特别是微球形式的支架，已在组织工程中被用来支撑细胞生长以及释放药物或生长因子。在本研究中，为了修复骨损伤，我们发展了一种制备支架方法，即根据其在丙酮中溶解性的差异，将ＰＨＢＶ微球包埋在ＰＬＧＡ基质中。ＰＬＧＡ／ＰＨＢＶ支架具有良好的孔参数，例如，ＰＬＧＡ／３０％ＰＨＢＶ支架的多孔性可达81.273 ± 2.192%。同时，我们也评估了这一模型的孔径分布，结果表明孔径主要分布在５０～ 200 μm范围。通过增加ＰＨＢＶ微球量，此高分子支架的抗压强度增强了。这种混合型支架的形貌别单纯的ＰＬＧＡ支架粗糙，而这一变化对细胞行为没有显著影响。体外评估表明这种模型适用于工程骨组织中的支架，并且在药物释放系统的进一步应用中具有很大潜力。 |
2楼2014-11-21 10:29:32