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The following abbreviations were
used to designate chemical shift mutiplicities: s = singlet, d
= doublet, t =triplet, m = multiplet, br = broad. Tumor cells
were cultured at 37C under a humidified atmosphere of
5% CO2 in RPMI 1640 medium supplemented with 10%
fetal serum and dispersed in replicate 96-well plates with
1910
4
cells per well. Complexes were then added. After
48 h exposure to the toxins, cell viability was determined
by the MTT colorimetric assay by measuring the absorbance at 570 nm with an ELISA reader. Each test was
performed in triplicate.

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篮球少年: 金币+10, 翻译EPI+1, ★★★★★最佳答案, 很不错 2014-11-20 17:45:31
        下面的简称用来指认化学位移峰的裂分状态:s为单峰,d为二重峰,t为三重峰,m为多重峰,br为宽峰。将肿瘤细胞分散在96孔板(每孔19104个细胞)中,于补充有10%的牛血清蛋白的RPMI 1640培养基中培养,并置于温度为37 C、CO2浓度为5%的潮湿气氛中。加入合成物后,让细胞暴露于这种毒素中48 h,然后采用MTT比色法,通过ELISA仪测定其在570 nm处的吸收,对细胞活性进行检测。每个实验重复3次。
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