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Phylogenic tree showing relationship between PM0525875 and Actinoalloteichus sp. based on clustering of the sequences of the 16S rRNA gene (coefficient of neighbour-joining) by using MEGA5 software There were a total of 1381 positions in the final datasheet. Evolutionaryanalyses were conducted on computer software MEGA5 . Based on this results the isolated actinomycetes strain was identified as Actinoalloteichus cyanogriseus. The gene sequence was deposited in NCBI GenBank under accession no KF861694.1. The antifungal active peak from the extract obtained by shake flask fermentation was identified by chromatographic and other analytical techniques during bioactivity guided isolation. Later the fermentation conditions were optimized in 30 L fermentor for the production of sufficient amount antifungal compound for complete structural characterization. Consequently the fermented broth extract was subjected to bioactivity-guided fractionation, to isolate the active principle using different preparative chromatographic techniques followed by its characterization.The active principle was characterized to be Caerulomycin A. |
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