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The absorption spectra of 2B-TPA in the water–DMF mixtures (5mM) are shown in Fig. 14. The spectral profile was markedly changed when >15% water was added to the DMF solution. The entire spectrum started to increase in absorption, indicating the formation of nanoscopic aggregates of 2B-TPA The light scattering, or Mie effect, of the nanoaggregates suspensions in the solvent mixtures effectively decreased light transmission in the mixture and caused the apparent high absorbance and level-off tail in the visible region of the UV absorption spectrum
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