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Diffusion Techniques
Quantitative methods that require measurement of zone diameters can also provide reproducible
estimates of the susceptibility of bacteria to antimicrobial compounds. Zone size provides an estimate of
the susceptibility of bacteria to antimicrobial compounds. The zone size should be determined using a
standard test method . This procedure uses paper disks impregnated with 30 mcg doxycycline to test
the susceptibility of bacteria to doxycycline. The disk diffusion interpretive criteria are provided in
Table 2.
Anaerobic Techniques
For anaerobic bacteria, the susceptibility to doxycycline can be determined by a standardized test
method . The MIC values obtained should be interpreted according to the criteria provided in Table 2.
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