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lirui901113

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We performed a renilla-luciferase assay with a modified expression pEZX vector containing the complete 3’ UTR region of PTEN cloned in the 3’UTR region of the dual luciferase gene. For dual-luciferase assay, 50 nM, 100 nM, 200 nM and 400 nM of the miR-19a, miR-21, miR-26a, miR-26b, miR-214, miR-1297 mimics and miRNC were transfected in the 293T cell line together with pEZX vector using lipofectamine 2000. 这是什么意思啊,谢谢!
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