| 查看: 900 | 回复: 3 | ||
| 本帖产生 1 个 翻译EPI ，点击这里进行查看 | ||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | ||
[求助]
求翻译，谢谢，站内信求大神帮忙
|
||
|
2.4. Selection of Natural Variant and Screening in Shake Flask The 5 ml of sterile tween 80 (0.1%) was added to fully sporulated slant. The spores were scraped with sterile cell scraper. The spore suspension was filtered through sterile cotton to remove the mycelial residues. The spore suspension was spread on AS-AIA. The plates were incubated at 30˚C for 30 days. The colonies having different morphology and sporulation pattern were picked up and transferred on AS-AIA slants. These variants were screened in shake flask as mentioned in 2.3. The harvested broth was extracted with equal amount of methanol and then serially diluted up to 1:1024 dilutions. Antifungal activities of this extract were checked by agar well diffusion assay [14]. |
回复此楼» 猜你喜欢
论文终于录用啦！满足毕业条件了
已经有10人回复
-
2025年遐想
已经有4人回复
-
投稿Elsevier的杂志（返修），总是在选择OA和subscription界面被踢皮球
已经有8人回复
-
求个博导看看
已经有18人回复
ssssllllnnnn 
至尊木虫 (知名作家)
Translator and Proofreader
- 翻译EPI: 1690
- 应助: 452 (硕士)
- 金币: 31580.9
- 红花: 100
- 帖子: 7681
- 在线: 19966.6小时
- 虫号: 3328089
- 注册: 2014-07-17
- 专业: 肿瘤发生
【答案】应助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
RXMCDM: 金币+1, 多谢应助！ 2014-10-26 12:26:54
bxzc123: 金币+10, 翻译EPI+1, ★★★很有帮助 2014-10-26 13:27:50
RXMCDM: 金币+1, 多谢应助！ 2014-10-26 12:26:54
bxzc123: 金币+10, 翻译EPI+1, ★★★很有帮助 2014-10-26 13:27:50
|
2.4. 自然变异体的选择的和在摇瓶中的筛选 向已经完全形成孢子的斜面培养基中加入5毫升无菌吐温80（0.1％）。用无菌细胞刮棒刮下孢子。孢子悬浮液通过无菌棉过滤，以除去菌丝体残留物。该孢子悬浮液涂布于AS-AIA。将平板置于30℃培养30天。挑取具有不同形态和孢子型式的菌落，并转移至AS-AIA斜面培养基。这些突变体的在摇瓶中的筛选用在2.3提到的方法进行。用等量甲醇提取所收获的培养液，然后连续稀释至1：1024倍。用琼脂孔扩散法[14]检测这种提取物的抗真菌活性。 |
2楼2014-10-26 10:36:45