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2.3. Secondary Screening of Active Isolate and Antifungal Activity
The isolate PM0525875 which exhibited exclusive antifungal activity was screened for its reproducibility as per
the procedure mentioned in 2.2. The methanolic extract of the whole culture broth, cell mass extract and cell free
culture filtrate were tested against Candida sp. to confirm the antifungal activity. To rule out the presence of
polyene in the active extract, the UV-visible spectrum of extract was compared with the UV-visible spectrum of
standard polyenes classes from the literature [15].
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RXMCDM: 金币+1, 多谢应助! 2014-10-26 12:27:41
bxzc123: 金币+20, 翻译EPI+1, ★★★很有帮助 2014-10-26 13:27:03
2.3. 活性分离物及其抗真菌活性的二次筛选
用2.2所提及的过程对具有独特抗真菌活性的分离物PM0525875进行筛选,以确定期重复性。用全培养液和细胞团的甲醇提取物,以及无细胞培养的滤液液对念珠菌属进行了测试以确定其抗真菌活性。为了排除活性提取物中存在多烯类,紫外 - 可见光光谱......
2楼2014-10-26 10:24:16
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