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Sodium pentobarbital (40 mg / kg) intraperitoneal injection of anesthesia, the method used to establish DooH.Lee modified rat model of spinal nerve ligation: supine fixed animals (anesthesia) in the animal operating table, back by the skin, disinfection shop towels , Touched on rats L4-S1 spike in the level of kin straight incision in the right skin, muscle separation, L6 until the right transverse process, Tichu its muscles and ligaments of attachment points, with the right to stop bleeding in addition to clamp bite L6 Transverse process, the glass from the right side Fenzhen L4, L5 spinal nerve, to avoid spinal nerve injury L4

Modeling for one hour before the oral gavage: left hand to seize control back and neck skin, skin and rat tail back fixed on the occasion of a big fish in the wrist, head and torso of straightening, and a vertical position. His right hand holding the syringe needles from oral infusion will be inserted, the needle will be inserted into the mouth to avoid teeth, along the posterior pharyngeal slowly into the lower esophagus, where resistance, it can gently sliding, once the feeling of resistance suddenly disappeared flu have come to nothing, Huichou syringe Die, without the bubbles out and animals without struggle, that infusion needle has entered the stomach. Slowly into the dimethylamine tetracycline (5 mg / ml) 3mg/100g or saline 1.2 ml/100g
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