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| 用pH 3.5 的磷酸氢二钠-柠檬酸缓冲液配制不同浓度的角豆胶底物溶液,在其他条件相同的情况下测定酶活性,利用Lineweaver-Burk 双倒数作图法求出酶的Km 及V max值。 |
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| The substrate solutions of locust beau gum with different concentrations were prepared with sodium hydrogen phosphate and citric acid buffer solutions of pH3.5, and the enzyme activity was detected under the same condition, the Km and Vmax was obtained with double reciprocal Lineweaver-Burk plot |
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