50楼: Originally posted by 花开满城香 at 2014-08-28 22:49:36
认为是这样的：用紫外扫描，在600纳米处有最大吸收峰，所以选择波长600纳米，又因为浓度值与OD值成正比，所以根据OD值可以算出浓度值，浓度值于细菌数成正比，最终OD值于细菌数成一定关系，有一个比例系数，希望能帮 ...

52楼: Originally posted by Jianfeng_Lin at 2014-08-30 05:15:20
As long as you have the standard curve(OD600-cell number) for a specific species, you can use the OD600 to get the rough cell number. To plot the standard curve, you need to test several OD600 of cel ...