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jiangjinlai木虫 (小有名气)
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[求助]
请各位师傅和老师帮忙翻译啊 化学英文到中文
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Example A.6 In 35.2 g(0.2mol)of 50% strength aqueous pyruvic acid are dissolved 6.5 g(0.1 mol) of metallic zinc powder.After the completion of hydrogen evilution,the mixture is stirred for a further a hours.Finally,the resultant white precipitate is filtered off with suction ,washed with 50 ml of water and 50 ml of methanol and dried at 50℃.and 15 mbar.The analyses clearly characterized the precipitate as zinc parapyruvate.The yield of zinc parapyruvate tetrahydrate is 24.0 g(77% of theory). Example B.1 Assays for Macrophage Activation and Cytokines A modified assay was carried out according to barbior et al.(B.M.Barbior,R.S.Kipnes,J.T.Curnutte,J.clin.Invest.,52,741,1973).In this assay 5×105 macrophages per titer and well were used in the presence of 10% fetl calf serum with increasing concentrations of zinc pyruvate (0-0.1-1×103 mM).The introduced number of macrophage cells corresponds to the release of HO2.orgate.being,or ensures that it is,exactly proportional to the concentration of the cell count.The blank value(blank,only buffer)and a negative control and a positive control (authentic substance,zinc pyruvate) were carried out such that specificity of the superoxide anions was ensured.Thus the wells were were inoculated with 15 微克 of superoxide dismutase at a cell count of 3.0×105 in order to act as a negative control. The macrophages were activated either with phorbol myristic ester(20微克/ml) or zymosan (100微克/ml).The macrophages thus stimulated were pretreated as negative control,blank or with the appropriate concentration of zinc pyruvate(authentic compound)(determination of cytoprotection) or added directly to the assay (determination of cytoprotection) or added directly to the assay (determination of inhibition),then washed with 0.02 M NaH2PO4 buffer(PH 6.5,20℃.)and then incubated with 0.5 ml of reaction mixture/Hank’s solution (phenol red-free),80 微M of ferricytochrome C (Sigma type IV) and 5 mM NaN3,which acts as a cytochrome oxidase inhibitor,and with stimulant at 37℃.for 20 min.Cytochrome C reduction was measured by the change in extinction at 550 nm.The superoxide anion concertration was determined via the difference in absorption at 550 nm in the presence or absence of supeoxide dismutase using the experimentally determined (working) extinction coefficient of 18.95/mM/cm(reduced or oxidized).The biochemical activities of inhibition of oxygen free radical formation were measured and evaluated in accordance with Michaelis-Menten kinetics. [search]sky[/search] |
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jiangjinlai(金币+5,VIP+0):谢谢
jiangjinlai(金币+5,VIP+0):谢谢
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NO分泌抑制剂的测定 用丙酮酸锌测定NO分泌作用的方法详见GRIESS反应(A.H.Ding,C.F.Nathan&D.J.Stuehr,J.Immunol.,141,2407,1988)。改进后的GRIESS试剂由以下组成：1 ml of 0.5%(g/g) naphthaleneethylenediamine H3PO4溶液，以击活巨噬细胞NO的释放，巨噬细胞(细胞计数:2.0×105)在加入75 units/ml IFN 的500 ul GRIESS试剂中 37℃下保温2小时，在加入20 ug/ml 的脂多糖(LPS,E.coli,MRE600)后，开始测定NO。此混合物在N2的保护下37℃下保温12小时。在NO生物合成正相和负相控制时，同时加入200 ug/ml的N6-乙基精氨酸和脂多糖，离心去除上清夜，加GRIESS试剂至适当体积，525 nm 光下5分钟之后萃取。 Naphthaleneethylenediamine没见过  第一个刚翻译完就看见不能回了,将就吧 |
2楼2008-04-15 13:18:00