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书名Atlas of PREIMPLANTATION GENETIC DIAGNOSIS 作者Anver Kuliev, MD, PhD Svetlana Rechitsky, PhD and Oleg Verlinsky, MS Reproductive Genetics Institute Chicago, Illinois, USA 页数324 出版社 CRCpress International Standard Book Number-13: 978-1-4665-9840-9 (eBook - PDF) Acknowledgments ii Preface v Introduction vi SECTION Ia•… Review of Current Methods and Experience in Preimplantation Genetic Diagnosis 1 Normal and abnormal human preimplantation development in relation to preimplantation genetic diagnosis and establishment of embryonic stem cell lines 1 Introduction 1 Oocytes at different stages of meiosis I 1 Oocytes at metaphase II and variations in first polar body formation 2 First polar body morphology in relation to chromosomal abnormality in oocytes 2 Oocytes following fertilization and second polar body extrusion 3 Normally and abnormally cleaving embryos 4 Normal and abnormal blastocyst formation 4 Establishment of human embryonic stem cell lines 5 References 5 2 Micromanipulation and biopsy of polar bodies, blastomeres, and blastocysts 7 First polar body removal 9 Intracytoplasmic sperm injection 10 First and second polar body removal 11 Blastomere biopsy 11 Blastocyst biopsy 12 Laser-assisted hatching 14 Trophectoderm biopsy 14 References 15 3 Nuclear transfer techniques for preimplantation diagnosis and prospect for artificial gamete formation 16 Visualization of polar body and blastomere chromosomes 16 Sperm duplication 18 Development of artificial human gametes in vitro 19 References 20 4 Preimplantation diagnosis for aneuploidies 21 Introduction 21 Preparation of polar bodies, blastomeres, and trophectoderm samples for fluorescence in situ hybridization analysis (FISH) 22 Preparation of PB1 and PB2 22 Preparation of blastomeres 25 Preparation of biopsied trophectoderm cells 25 Pretreatment, probe application, hybridization, and washing 26 Hybridization procedure 26 Washing procedures 27 Fluorescent signal evaluation 28 Array-based 24-chromosome aneuploidy testing 29 Chromosomal abnormalities in polar bodies 29 Chromosomal abnormalities in cleaving embryos and blastocysts 30 References 31  (Encyclopedia of visual medicine series.jpg |
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