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shji03zhangjing

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[×ÊÔ´] ÓйØMTT·½ÃæµÄһЩ֪ʶ

MTT????????áé????????????????????

MTT??????????????§Ý?????3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide, MTT?????????????MTT????????????????????????????????????????????????§Ö?????????????????????????????C????????tetrazolium????????????????formazan????formazan????????????????????????????????????????????????????????MTT???????????????formazan???????50%??N,N-?????????????20%???????????????pH 4.7????MTT???????????????????490 nm????????OD??????????????????????????DMSO?????
MTT??????????????????????????????????????????????????????????????????????????????¨¢?

MTT

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1????????????¨²?10???§³?????????????????????????????
1000??10000??????????96??ÈÉ??????200ul.

2:???????????????????????????3??5??????????????????????????????

3???????????3??5???????MTT?????5mg/ml??PBS ??)20ul.
????????4§³????????????§³?????????????????????????????????????????????????????????????????150ul DMSO????10???????????????

4??????????490nm?????????????????????????????????????????????????????????????????????????????????

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??1?????????????????????
??2?????????????????§³??10??????????????????????î•??????????????????????????
??3??????????????????§Ó?????????????????????????????öø?Øb??????????????????

MTT??????????????0-0.7????????????¦¶????????????

IC50?????????????????????50???????????????????????????????????????????????????????????????????????????????????????50??????????????????????IC50???????2??????????????????????????

???????????????0.1??0.01??0.001??0.0001??0.00001??0.000001?????????10?????????0.1?????????0.95?? 0.80??0.65??0.43??0.21??0.06??????
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Pm=0.95
Pn=0.06
P=0.95 0.80 0.65 0.43 0.21 0.06=3.1
Xm=lg0.1=-1
lgI=lg0.1/0.01=1
lgIC50=-1-1*(3.1-(3-0.95-0.06)/4)=-3.6025
IC50=0.00025

????????????¦Ï?;
lgIC50=Xm-I(P-(3-Pm-Pn)/4)
Xm:lg ??????
I:lg(??????/???????)
P:???????????
Pm:???????????
Pn:??§³????????

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??????4000???????????????????20000??/ml??MTT??20ul????????§³??????????????????????????????????150ul DMSO??????????????10??????????????
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shji03zhangjing

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2Â¥2008-04-09 15:24:06
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jia0436

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ллÁË,ÎÒ×î½üÒ²ÊÇÔÚ×öMTT,¸Õ¿ªÊ¼×ö,Çë¶àÖ¸½Ì.
4Â¥2008-04-11 18:33:00
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taotao4108

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