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北京石油化工学院2026年研究生招生接收调剂公告
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yyc12596

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[交流] microvesicle ultracentrafuge 已有1人参与

microvesicle获取。
   文献中有用AnnexinV磁珠拉下来的。
    但是据说ultracentrafuge也可以。对于超声离心不熟悉,查查资料看看吧。
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yyc12596

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Senescence-associated release of transmembrane proteins involves proteolytic processing by ADAM17
and microvesicle shedding
Timo Effenberger,*,1 Jan von der Heyde,*,1 Kareen Bartsch,* Christoph Garbers,*
Klaus Schulze-Osthoff,†,‡,§ Athena Chalaris,* Gillian Murphy, Stefan Rose-John,*
and Björn Rabe*,2
6楼2014-08-04 23:44:47
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yyc12596

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★ ★ ★
youlinglyw: 金币+3, 赠人玫瑰手有余香,生物科学有你更精彩! 2014-08-05 09:42:24
实验室的Ultracentrifuge是Beckman Coulter的Optima™ L-80 XP。
我之前没用过。

公司对于仪器的说明网址:
https://www.beckmancoulter.com/w ... /2/392049///0/1//0/
2楼2014-08-04 23:31:26
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yyc12596

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配套的软件:
Optima's easy-to-use eXPert Software gives your lab an edge by making it possible to quickly design and optimize separation protocols using a combination of rotors and tubes to simulate results — all before performing separations. A unique slide-bar feature allows you to view the separation at earlier run times to better suit the isolation of individual components. Up to three components can be modeled using the rate-zonal protocol. Then save the simulated run and download it with a single click of the mouse when you're ready to begin your run. Optima eXPert Software's advanced intelligence delivers greater productivity and faster turnarounds for your lab.

The following demonstrations illustrate just how easy it is to optimize efficiency with Optima eXPert Software.
3楼2014-08-04 23:34:31
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yyc12596

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设置时间:
Optima eXPert Software Demo 2:
Run Simulation -- Rate Zonal

Find out how to stop your run at the optimum separation time before the sample material reaches the tube bottom and becomes a pellet.
4楼2014-08-04 23:35:51
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