| 查看: 1146 | 回复: 6 | |||
| 【奖励】 本帖被评价6次，作者rockyss增加金币 4.2 个 | |||
[资源]
【推荐】 JACS和scientific report报道-可控自组装蛋白纳米管
|
|||
|
利用分子伴侣GroEL的天然双环结构自组装成纳米管，可用于生物医药领域。。 文献制备方法简单，表征手段前沿，可以作为蛋白纳米材料方向研究生的参考资料 1. Self-assembly of the chaperonin GroEL nanocage induced at submicellar detergent Scientific Reports http://www.nature.com/srep/2014/ ... full/srep05614.html Volume: 4,Article number:5614 DOI: doi:10.1038/srep05614 Published08 July 2014 Protein nanoassemblies possess unique advantage in biomedical applications such as drug delivery, biocatalysis and vaccine development. Despite recent accomplishment in atomic structure data, the underlying molecular mechanism of protein self-assembly remains elusive, where considerable heterogeneity is often involved. Here we use E. coli chaperonin GroEL, a tetradecameric protein with a molecular weight of 805 kDa, to probe its transformation from cage-like oligomers to protein nanofibers. We show that sodium dodecyl sulfate (SDS), a widely-used protein denaturant, at submicellar concentration binds to and causes partial distortion of GroEL apical domain. Subsequently, the GroEL apical domain with altered secondary structural content converts the GroEL oligomers into modular structural units which are observed to self-assemble into cylindrical nanofibers under an agitated incubation in a physiological buffer. Interestingly, through targeted mutagenesis where two cysteine residues are introduced at the entry site of GroEL cage, we found that the formation of GroEL nanoassembly could be modulated depending on the redox condition of incubation. Without the need of chemical engineering, tunable GroEL nanofibers built by controlled-assembly are among the largest nanoscale bioassembly with broad applications.  2. A Tubular Biocontainer: Metal Ion-Induced 1D Assembly of a Molecularly Engineered Chaperonin J. Am. Chem. Soc., 2009, 131 (22), pp 7556–7557 DOI: 10.1021/ja902696q Publication Date (Web): May 12, 2009 http://pubs.acs.org/doi/abs/10.1021/ja902696q GroELSP/MC, prepared by genetic and chemical modifications of group I chaperonin protein GroEL, site-specifically possesses 28 photochromic (spiropyran [SP] and merocyanine [MC]) units in the entrance parts of its cavity. Addition of divalent metal ions such as Mg2+ to a tris-HCl buffer solution of GroELSP/MC results in one-dimensional (1D) assembly of GroELSP/MC, affording cylindrical hollow fibers with a very large aspect ratio; the longest fiber was 2.5 μm long, corresponding to a 170-mer of GroELSP/MC (MW ≈ 1.4 × 108). When such long fibers are mixed with EDTA, they are cut into short-chain oligomers and eventually into monomeric GroELSP/MC. Similar to GroEL, GroELSP/MC possesses a large binding affinity toward denatured proteins. When GroELSP/MC undergoes 1D assembly after incubation with a denatured protein, guest-containing cylindrical fibers result.  |
回复此楼» 本帖附件资源列表
-
欢迎监督和反馈：小木虫仅提供交流平台，不对该内容负责。
本内容由用户自主发布，如果其内容涉及到知识产权问题，其责任在于用户本人，如对版权有异议，请联系邮箱：xiaomuchong@tal.com - 附件 1 : 2014_srep05614.pdf
- 附件 2 : 2009_JACS.pdf
2014-07-30 23:44:53, 6.2 M
2014-07-30 23:45:13, 1.41 M
» 收录本帖的淘帖专辑推荐
 生物材料类型 |
» 猜你喜欢
一志愿暨大生物学290求求调剂
已经有8人回复
-
厦大309分生物与医药调剂
已经有0人回复
-
生物力学与组织工程学论文润色/翻译怎么收费?
已经有50人回复
-
334生物求调剂
已经有0人回复
-
一志愿中山大学生物学，295分，求A区调剂
已经有2人回复
-
求助化合物CD数据的处理
已经有0人回复
-
广州医科大学生物医学工程学院 生物医学工程工学-083100 招生
已经有5人回复
-
一志愿湖南大学 生物学332分 有国奖+省级
已经有1人回复
-
美国南加大(USC)药学院Prof. Yong (Tiger) Zhang课题组诚聘博士后
已经有0人回复
-
湖北工业大学食品科学与工程系课题组招收2026级食品/生物医药硕士生（含调剂）
已经有0人回复
» 本主题相关商家推荐: (我也要在这里推广)
» 本主题相关价值贴推荐，对您同样有帮助:
- 高分子专业相关期刊介绍【转】
已经有22人回复
- 生物材料版资源帖汇总
已经有20人回复
- 【sobereva个人文集】乱弹DFT-D（从sobereva的博客中转载过来的）
已经有27人回复
- 【原创】公派读博的准备经历的一点总结
已经有90人回复
- 【招聘】撤了
已经有7人回复
- 【招聘信息】撤了
已经有4人回复
- 【原创】新加坡李光耀博士后奖学金介绍
已经有16人回复
- 【推荐】@#￥想成为化学达人的一定要经常进来看看~~
已经有14人回复
- 【推荐】有机化学常用期刊网址，强烈推荐哦
已经有10人回复
- 【分享】有机化学类期刊点评:牛刊一览
已经有69人回复
- 南京大学长江学者团队诚聘纳米线电子学、染敏太阳电池、宽禁带半导体器件方向博士后
已经有33人回复
- 【原创】【分享】离子液体综述大全，带你进入离子液体的世界！（下载地址已更新）
已经有182人回复
4楼2014-08-03 03:04:25
简单回复
2014-08-02 02:16
回复
五星好评 顶一下，感谢分享！
2014-08-03 01:29
回复
五星好评 顶
lwg35楼
2014-08-03 09:43
回复
五星好评 顶一下，感谢分享！
2014-08-09 00:12
回复
三星好评 顶一下，感谢分享！
zhouxj7楼
2014-10-01 21:48
回复
五星好评 顶一下，感谢分享！
