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holiland

新虫 (初入文坛)

[求助] 请问这个Ampligase是连接酶吗?具体是什么酶?

请问这个Ampligase是什么酶?是连接酶吗?

原文是这样的
Complex three-dimensional MDA amplicons were reduced to linear DNA using DNA polymerase I and Ampligase. This process substantially
improved the complexity of the library during sequencing.
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至尊木虫 (知名作家)

Translator and Proofreader


【答案】应助回帖

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RXMCDM: 金币+1, 多谢应助! 2014-07-24 00:15:12
holiland(RXMCDM代发): 金币+5, 多谢应助! 2014-08-08 15:35:57
RXMCDM: 翻译EPI+1 2014-08-08 15:36:13
Ampligase® Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3´-hydroxylated and 5´-phosphorylated termini in duplex DNA structures that are stable at high temperatures. Derived from a thermophilic bacterium, the enzyme is stable and active at much higher temperatures than conventional DNA ligases.

为一种耐热性DNA连接酶,在高温条件下催化NAD依赖性连接(DNA双链之3‘羟基端与5’磷酸基团的连接)。由耐热菌获取。该酶在较高温度条件下比常规DNA连接酶稳定且具活性。
2楼2014-07-23 21:08:08
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