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[求助] 菌种鉴定中DNA-DNA杂交实验关键点,求大神指点,准备微量稀释板做

最近要做菌种分类,全基因组测序太贵了,准备用这个DNA-DNA杂交试验来代替,看了点儿文献觉得有几处关键点,求大神指正1
1.提取DNA的量,包被和做标记DNA的时候文献对其浓度都有具体要求,我准备用试剂盒替代,不知道是否可行
2.对包被的原理有些疑惑,有的是通过对DNA处理使其产生共价键来完成包被,但有的直接是在电解缓冲液中完成包被,求解释
3.有包被肯定得有封闭,但为什么在杂交的时候还要SAMON DNA?
这是所有家当,真诚求助
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