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yaoguiyang木虫 (小有名气)
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[求助]
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The growth of stem cells can be modulated by physical factors such as extracellular matrix nanotopography. We hypothesize that nanotopography modulates cell behavior by changing the integrin clustering and focal adhesion (FA) assembly, leading to changes in cytoskeletal organization and cell mechanical properties. Human mesenchymal stem cells (hMSCs) cultured on 350 nm gratings of tissueculture polystyrene (TCPS) and polydimethylsiloxane (PDMS) showed decreased expression of integrin subunits a2, a6, aV, b2, b3 and b4 compared to the unpatterned controls. On gratings, the elongated hMSCs exhibited an aligned actin cytoskeleton, while on unpatterned controls, spreading cells showed a random but denser actin cytoskeleton network. Expression of cytoskeleton and FA components was also altered by the nanotopography as reflected in the mechanical properties measured by atomic force microscopy (AFM) indentation. On the rigid TCPS, hMSCs on gratings exhibited lower instantaneous and equilibrium Young’s moduli and apparent viscosity. On the softer PDMS, the effects of nanotopography were not significant. However, hMSCs cultured on PDMS showed lower cell mechanical properties than those on TCPS, regardless of topography. These suggest that both nanotopography and substrate stiffness could be important in determining mechanical properties, while nanotopography may be more dominant in determining the organization of the cytoskeleton and FAs. |
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reko34
木虫 (正式写手)
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RXMCDM: 金币+1, 多谢应助！ 2014-06-19 12:38:14
yaoguiyang: 金币+40, 翻译EPI+1, ★★★★★最佳答案 2014-06-19 13:12:17
RXMCDM: 金币+1, 多谢应助！ 2014-06-19 12:38:14
yaoguiyang: 金币+40, 翻译EPI+1, ★★★★★最佳答案 2014-06-19 13:12:17
| 可通过例如细胞外基质纳米形貌的物理因素调节干细胞的生长。我们假设纳米形貌通过改变整合素聚集和粘着斑（FA）集合调节细胞行为，导致细胞骨架组织和细胞机械性能变化。与非模式化的控制相比，在组织培养聚苯乙烯(TCPS)和聚二甲基硅氧烷（PDMS）的350nm格栅上培养得到的人间充质干细胞（hMSCs）上，整合素的子成分a2、a6、aV、b2、b3和b4的出现减少。格栅上，延长了的hMSCs展现出对齐的肌动蛋白细胞骨架，而在非模式化控制下，扩散细胞展现出随机但较密的肌动蛋白细胞骨架网络。细胞骨架和FA成分的表现度也被纳米形貌改变，如原子力显微镜(AFM)测量的机械性能所反映的。在刚性TCPS上，格栅上的hMSCs显示出较低的瞬态和稳态杨氏模量及明显的粘性。在较软的PDMS上，纳米形貌的作用不显著。但PDMS上培养的hMSCs与TCPS上的相比，细胞机械性能较低，不论地貌如何。这些结果显示，纳米形貌和基板硬度都对机械性能起到重要作用，其中纳米形貌在决定细胞骨架和FA组织上起主导作用。 |
2楼2014-06-19 11:12:14
yaoguiyang
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3楼2014-06-19 13:36:07
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