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新虫 (小有名气)

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[求助] 为什么要肝门静脉取血已有4人参与

今天看了一篇代谢组学的文献，研究一方剂对肝的治疗作用，采用肝门静脉取血，
为什么呀？避免首过效应？这样对代谢物有什么影响？
和眼眶、颈动脉相比有什么特点啊

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1楼 2014-06-11 16:55:16

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17楼: Originally posted by richbird at 2014-06-19 17:34:23
代谢组学会受吸收药物和药物代谢的双重影响，蛋白组学受到的影响相对较少。因为是复方，化合物成分复杂。但是对于多数药物而言，会结合在血浆蛋白上。当然组织器官也有有一定的蛋白结合，假如没有特异性结合或者分布 ...

把方法的英语原文贴出来吧，避免英语理解错了的原因

Animal sampling and handing
The animals were randomly assigned to 10 groups of 6 rats each as follows: control, HI, YCHT, D, G, R, DG, DR, GR and DGR groups. The rats in the control group were given distilled water for 8 consecutive days and anesthetized via an intraperitoneal injection of 1% pentobarbital sodium (0.15 ml/100 g body weight) on day 9. The rats in the HI group were orally administrated 20% CCL4 (1 ml/kg body weight) for 8 consecutive days (at 6:00 p.m.), and on day 9, the rats were anesthetized as in the control group. Simultaneously, other groups were administrated CCL4 and various treatments for 9 consecutive days, then anesthetized as in the control group. The rats were orally given 1 ml/100 g each, and the doses of the three components used in this study were administered in accordance with the “Shanghanlun”. Blood was collected from the hepatic portal vein, and plasma was separated via centrifugation at 4,500 rpm for 5 min at 4°C, flash frozen in liquid nitrogen and stored at -80°C until the liver function tests and metabolomics and proteomics analyses were performed. The plasma ALT, AST, ALP, GSH-PX and SOD activities and MDA, TG, GT, CHOL, TP, D-BIL and T-BIL contents were measured according to the manufacturer’s instructions. The rat livers were removed immediately after plasma collection and stored at -70°C until analysis. Urine was collected daily (at 6:00 a.m.) from the metabolic cages at ambient temperature throughout the entire procedure and centrifuged at 10,000 rpm at 4°C for 5 min; the supernatants were then stored frozen at -20°C for subsequent metabolomic analysis.