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Gao S, Li Y, Zhang J, Chen H, Ren D, Zhang L, An Y. A modified version of the digestion-ligation cloning method for more efficient molecular cloning. Anal Biochem. 2014 453: 55-57. [Epub ahead of print]
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A modified version of the digestion-ligation cloning method for more efficient molecular cloning
×÷Õß:Gao, S (Gao, Song)[ 1 ] ; Li, YL (Li, Yanling)[ 1 ] ; Zhang, JN (Zhang, Jiannan)[ 1 ] ; Chen, HM (Chen, Hongman)[ 1 ] ; Ren, DM (Ren, Daming)[ 1 ] ; Zhang, LJ (Zhang, Lijun)[ 1 ] ; An, YF (An, Yingfeng)[ 1 ]
ANALYTICAL BIOCHEMISTRY
¾í: 453  Ò³: 55-57
DOI: 10.1016/j.ab.2014.02.031
³ö°æÄê: MAY 15 2014
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Here we describe a modified version of the digestion-ligation approach for efficient molecular cloning. In comparison with the original method, the modified method has the additional steps of gel purification and a second ligation after the first ligation of the linearized vector and DNA insert. During this process, the efficiency and reproducibility could be significantly improved for both stick-end cloning and bluntend cloning. As an improvement of the very important molecular cloning technique, this method may find a wide range of applications in bioscience and biotechnology. (C) 2014 Elsevier Inc. All rights reserved.
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×÷Õ߹ؼü´Ê:Blunt-end DNA ligation; T4 DNA ligase; Molecular cloning; Digestion-ligation approach
KeyWords Plus:INDEPENDENT CLONING; PCR PRODUCTS; ENZYME-FREE; DNA; LIGASE
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ͨѶ×÷ÕßµØÖ·: An, YF (ͨѶ×÷Õß)
              Shenyang Agr Univ, Coll Biosci & Biotechnol, Shenyang 110161, Peoples R China.
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              [ 1 ] Shenyang Agr Univ, Coll Biosci & Biotechnol, Shenyang 110161, Peoples R China
µç×ÓÓʼþµØÖ·:anyingfeng666@163.com
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National Natural Science Foundations of China        
31100045
31270114
Program for Liaoning Excellent Talents in University        
LJQ2011067
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ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
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Ñо¿·½Ïò:Biochemistry & Molecular Biology; Chemistry
Web of Science Àà±ð:Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical
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ÎÄÏ×ÀàÐÍ:Article
ÓïÖÖ:English
Èë²ØºÅ: WOS:000335488400010
ISSN: 0003-2697
µç×Ó ISSN: 1096-0309
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IDS ºÅ: AG5WC
Web of Science ºËÐĺϼ¯ÖÐµÄ "ÒýÓõIJο¼ÎÄÏ×": 15
Web of Science ºËÐĺϼ¯ÖÐµÄ "±»ÒýƵ´Î": 0
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