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小钱不帅铁杆木虫 (正式写手)
奋青
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[求助]
求大神帮助小弟翻译一下生物论文摘要，急啊
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从土壤中分离得到一株对一些病原菌具有抑制作用的菌株，并将其命名为BLy。该菌株能够产生多种生物活性物质，并对金黄色葡萄球菌具有抑制作用。通过对菌株BLy的16S rDNA 的序列测定和同源性比对，以及结合形态和生理生化指标，将其鉴定为地衣芽孢杆菌（Bacillus licheniformis）。该菌在医药、饲料加工、农药等领域都有着广阔的应用前景。 本论文以LB培养基对地衣芽孢杆菌BLy进行摇瓶发酵，提取不同时间段的发酵液，经过离心及过滤获得无菌发酵液，以受其抑制作用较为明显的金黄色葡萄球菌作为指示菌，用平板抑菌圈法检测BLy菌株产抑菌物质发酵曲线。用根霉菌、酵母菌、毛霉菌、青霉菌、红酵母、黑曲霉、大肠杆菌、金黄色葡萄球菌及枯草芽孢杆菌作为指示菌株，测定其抑菌谱，得出该菌所产抑菌物质主要对革兰氏阳性细菌金黄色葡萄球菌、枯草芽孢杆菌具有较好的抑制效果，对革兰氏阴性细菌大肠杆菌抑制作用不明显，对所给真菌无抑制作用。 |
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RXMCDM
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小钱不帅: 金币+10, ★有帮助 2014-06-08 22:06:16
小钱不帅: 金币+10, ★有帮助 2014-06-08 22:06:16
| One soil-isolated and pathogen bacterial strain was name after Bly ,which can produce various bioactive substance and has an antibacterial effect on Staphylococcus aureus . This strain was identified as Bacillus licheniformis through 16S rRNA gene sequence analysis homology comparison ,together with bacteria morphology and the physiological and biochemical indexes 。The bacteria has a broad application prospect in such field as medicine, pesticide, and feed processing. Therefore,the Bly fermentation curve of antibacterial substance producing was tested by Bacillus licheniformis shake flask culture on LB medium ,in which the fermentation liquid was extracted at different time interval and asepticized through centrifugation and filtration to detect the antibacterial substance by inhibition zone detection with effectively obvious Staphylococcus aureus as indicator .The Bly antimicrobial spectrum was also detected by the use of indicating bacterium of Rhizopus, Saccharomyces, Mucor, Penicillium, Rhado-torula, Aspergillus niger, Escherichia coli, Staphylococcus aureus and Bacillus subtilis,which revealled that the produced antibacterial substances had better effect on gram-positive bacteria such as Staphylococcus aureus and Bacillus subtilis,no obvious effect on gram-negative bacteria of Escherichia coli,and no effect on the tested fungus. |
7楼2014-06-08 18:07:06
Paulwolf
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2楼2014-06-08 09:57:19
Luse_91
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真强必胜: 金币+1, ok 2014-06-08 10:55:04
RXMCDM: 金币+1 2014-06-08 18:07:41
小钱不帅: 金币+20, ★★★很有帮助 2014-06-08 22:03:38
真强必胜: 金币+1, ok 2014-06-08 10:55:04
RXMCDM: 金币+1 2014-06-08 18:07:41
小钱不帅: 金币+20, ★★★很有帮助 2014-06-08 22:03:38
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第一段翻译： The strains separated from soil have inhibitory effect on some pathogenic, and which are defined as Bly. The strain was able to produce various bioactive substances,and has inhibitory effect on Staphylococcus aureus. It was identified as Bacillus licheniformis through the determination and homology comparison the sequence of the strain BLy 16S rDNA, as well as the combination of morphological and physiological and biochemical indexes. The bacteria has a broad application prospect in the fields of medicine, pesticide, feed processing. |
3楼2014-06-08 10:00:25
Luse_91
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RXMCDM: 金币+1 2014-06-08 18:07:35
RXMCDM: 金币+1 2014-06-08 18:07:35
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第二段： In the paper, the LB culture medium for Bacillus licheniformis BLyshake flask fermentation, the fermentation liquid extraction in different time, after centrifugation andfiltration to obtain sterile broth, with its inhibitory effect was obvious in Staphylococcus aureus as indicator bacteria, with flat bacteriostatic circle method to detect the fermentation curve of the antibacyerial substance produced by Bly strains. With Rhizopus, Penicillium, Mucor, Yeast, Rhodotorula glutinis, Aspergillus niger, Escherichia coli, Staphylococcus aureus and Bacillus subtilis as indicator strains, through determining of the antibacterial spectrum, the antibacterial substances produced by the strains mainly had better inhibition effect on gram positive bacteria, Staphylococcus aureus, Bacillus subtilis, had no obviously inhibitory effect on the gram negative bacteria Escherichia coli, also had no inhibition effect on the fungi. |
4楼2014-06-08 10:18:27
