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紧急求助：劳驾色谱高手进来看看，关于ATP ADP AMP的问题
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紧急求助：劳驾色谱高手进来看看，关于ATP ADP AMP的问题 花了老板两千多块钱 买了SIGMA公司三个标准品 准备做HPLC SIGMA公司 Adenosine 5`-diphosphate Agarose ( ADP ) 货号 A2810 规格:1ml Catalog Number A2810 Matrix cross-linked 4% beaded agarose Mtx activatn: cyanogen bromide Mtx atchmt: C-8 Mtx spacer: 9 atoms Adenosine 5`-monophosphate Agarose ( AMP ) 货号3019 规格:1ml lyophilized powder, Contains lactose stabilizers that must be removed prior to use. MDL number MFCD00801331 Descriptions Physical form Lyophilized powder stabilized with lactose Legal Information Sepharose is a registered trademark of GE Healthcare. Properties form lyophilized powder extent of labeling 2-10 μmol per mL matrix cross-linked 4% beaded agarose matrix activation cyanogen bromide matrix attachment N-6 matrix spacer 8 atoms storage temp. −20°C Safety WGK Germany 3 Related Categories ... Affinity Chromatography > Nucleotide/Coenzyme Resins Adenosine 5`-triphosphate Agarose ( ATP ) 货号 A2767 规格:1ml Descriptions Physical form Lyophilized powder stabilized with lactose Legal Information Sepharose is a registered trademark of GE Healthcare. Properties form lyophilized powder extent of labeling 1-5 μmol per mL matrix cross-linked 4% beaded agarose matrix activation cyanogen bromide matrix attachment C-8 matrix spacer 9 atoms storage temp. −20°C Safety WGK Germany 3 Related Categories ... Essential Biological Reagents > Affinity Resins ... Affinity Chromatography > Nucleotide/Coenzyme Resins 后来发现 这个药品不能以水溶解 标签上说明是将药品以水或缓冲液溶解后过微孔滤膜 我们照做了 将它处理以后进液相色谱 可好像没有发现一个像样的峰出来 出来的峰都很小 觉得像杂质 而非我们想要的东西 后来一查资料发现 那个东西 是亲和色谱制备的 有没有哪位高手能指点一二啊 亲和色谱是怎么回事儿？这个药品该怎么用呢？能不能用在HPLC上呢？ |
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