Hepatic expression of CYP isoforms including CYP1A2 (Fig. 3A), 1B1 (Fig. 3B), 2B1 (Fig. 3C), 2C11 (Fig. 3D), 2E1 (Fig. 3E), 3A1
(Fig. 3F), 3A2 (Fig. 3G), and 4A1 (Fig. 3H), were measured in rats treated with SHT extracts twice a day for 1 week. Hepatic expression
of CYP1A2, 1B1, 2B1, 2C11, 3A1, 3A2, and 4A1 did not change with SHT extract treatment. In addition, hepatic CYP1A1 protein
was not detectable in both normal control rats and SHT-treated rats (data not shown). Interestingly, the expression and activity
of CYP2E1 were significantly increased in SHT-con (P < 0.005), SHT-LF (P < 0.005), and SHT-LG (P < 0.001) rats relative to normal
ontrol rats, by 1.60-, 1.65-, and 1.87-fold respectively (Fig. 3E).
关于这个结果图,论文中是这样描述的。CYP2E1活性显著增加了。其他的没有变化。可是从图上看的话,CYP2E1的NC(control)是100%,SHT-con是150%多。而CYP1B1和CYP2B1,CYP4A1的增加量也不少呀。。。而且为啥每个条形图上面Western Blot的色带有两个呀。。名字都一样。。
论文全文在附件里。
救命啊。。。求解答。。不胜感激![救命,关于CYP450,菜鸟看论文看不懂来求助。]()
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![救命,关于CYP450,菜鸟看论文看不懂来求助。-1]()
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