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Nature出版社旗下方法学专刊Focus on Synthetic Biology
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Focus issue: May 2014 Volume 11, No 5 Synthetic biology spans many disciplines and has many different goals. A Commentary discusses its role in addressing fundamental questions such as the origin of life. Although recent advances in the field have brought us closer to some of synthetic biology's ambitious aims, there is still a need for better characterization of basic building blocks. One Review looks at advances and challenges in DNA synthesis and error correction, a second Review discusses optimal genetic circuit design, and a research paper presents a computational tool for efficient assembly of genetic parts. A Perspective highlights advances in whole-genome assembly and activation. Well-characterized tools and methods will allow the field to move from costly trial-and-error approaches to a more standardized plug-and-play approach. EDITORIAL Focus on Synthetic Biology Synthetic Biology: Back to the Basics - p463 Nicole Rusk doi:10.1038/nmeth.2941 COMMENTARY Focus on Synthetic Biology A synthetic approach to abiogenesis - pp495 - 498 James Attwater & Philipp Holliger doi:10.1038/nmeth.2893 Authors discuss how synthetic biology approaches could be applied to assemble synthetic quasibiological systems able to replicate and evolve, illuminating universal properties of life and the search for its origins. REVIEWS Focus on Synthetic Biology Large-scale de novo DNA synthesis: technologies and applications - pp499 - 507 Sriram Kosuri & George M Church doi:10.1038/nmeth.2918 This Review discusses large de novo DNA synthesis via oligos or arrays, describes gene assembly and error correction and considers applications for large-scale DNA synthesis. Focus on Synthetic Biology Principles of Genetic Circuit Design - pp508 - 520 Jennifer A N Brophy & Christopher A Voigt doi:10.1038/nmeth.2926 This Review introduces tools to build transcriptional circuits and explains how the choice of different tools can affect circuit behavior and how its operation can be affected by the cellular host. PERSPECTIVE Focus on Synthetic Biology Programming biological operating systems: genome design, assembly and activation - pp521 - 526 Daniel G Gibson doi:10.1038/nmeth.2894 This Perspective takes the reader through the important steps in bacterial genome assembly and activation and concludes with an outlook on how customized genomes may be achieved. ARTICLE Focus on Synthetic Biology Interactive assembly algorithms for molecular cloning- Evan Appleton, Jenhan Tao, Traci Haddock & Douglas Densmore doi:10.1038/nmeth.2939 Raven calculates assembly plans for complex genetic constructs from thousands of parts. It integrates user feedback on failed intermediate assemblies to improve the final outcome. http://pan.baidu.com/s/1ntI6faD  QQ截图20140521231618.png |
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