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三七残渣中三七素的提取纯化及含量测定 摘要： 目的 检测云南文山三七经皂苷提取后的残渣中是否存在三七素，以及对其进行系统系的初步提取纯化和含量测定。 方法 采用水提、离心、浓缩、醇沉获得三七素提取物，通过薄层色谱检测，一高效液相色谱进行含量测定。 结果 三七残渣中检测出三七素的存在，且三七素在三七残渣中的含量为______，初步可提纯浓度为_____，在2～80 μg/ml范围内线性关系良好(r=0.9995)，标准曲线为y = 9655.9x + 35202，平均回收率为______%，RSD为______。 结论 三七残渣中存在三七素，可初步对其进行提取纯化和含量测定。 |
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【答案】应助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
RXMCDM: 金币+1 2014-05-19 20:12:58
周羽2011: 回帖置顶 2014-05-22 15:53:30
周羽2011(phu_grassman代发): 金币+25, thanks. 2014-05-27 12:10:08
RXMCDM: 金币+1 2014-05-19 20:12:58
周羽2011: 回帖置顶 2014-05-22 15:53:30
周羽2011(phu_grassman代发): 金币+25, thanks. 2014-05-27 12:10:08
| Objective To detect the dencichine in the residual of extracts from Yunnan Wenshan panax notoginseng and to purify and determine the content of dencichine.Method Dencichine were obtained through water extracting, centrifugation, concentrating and precipitation by ethanol, decichine was detected by TLC and determined the content by HPLC. Result Dencichine was detected in the Panax notoginseng residual with the content of . the concentration of the preliminary extracting was . the calibration curve was linear over the range of 2～80 μg/ml (r=0.9995)，the standard curve was y = 9655.9x + 35202, The average recovery rate was % with % of RSD(n=).conclusion Decinchine was detected in the residual after extracting from Panax notoginseng and was purified and determined. |
4楼2014-05-19 16:33:37
【答案】应助回帖
★
爱与雨下: 金币+1 2014-05-18 08:17:47
RXMCDM: ？？？？？？ 2014-05-18 14:54:19
phu_grassman: 金币-2, bad try 2014-05-27 12:09:21
爱与雨下: 金币+1 2014-05-18 08:17:47
RXMCDM: ？？？？？？ 2014-05-18 14:54:19
phu_grassman: 金币-2, bad try 2014-05-27 12:09:21
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37 vegetables withdraw to purely turn in 37 remnants and content measurement Abstract:The purpose examines text mountain in Yunnan 37 after soap Mao withdraws of remnant in whether exist 37 vegetable, and as to it's the first step carrying on system to fasten withdraw to purely turn to measurese with content.Method adoption water lifts, leaves heart, condense, the Chun sink to acquire 37 vegetables to withdraw a thing and pass a thin layer color table examination, an efficiently liquid mutually the color table carry on a content measurement.Examine the existence of 37 vegetables in result 37 remnants, and 37 vegetables at 37 contents in the remnant is______, the first step can lift a pure density for_____, in 2~80 μ gs|ml scope the inside track contains good sexual behavior(r=0.9995), standard curve is y=9655.9 xes+35202, average recovery rate is______%, RSD is______.Exist 37 vegetables in conclusion 37 remnants, initial as to it's carry on withdrawing to purely turn to measurese with content. |
2楼2014-05-17 18:12:15
3楼2014-05-19 13:24:11
