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有谁知道Masson‘s trichrome stain即masson三色染色、马氏三色染色法的原理和意义。
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我想要知道masson三色染色的原理和意义、主要是用在什么实验当中。谢谢大家。 [ Last edited by hshzhq on 2008-3-12 at 22:26 ] |
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dajiahaoma
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asr(金币+3,VIP+0)::P 非常感谢，欢迎常来交流！辛苦了
hshzhq(金币+1,VIP+0):谢谢了，不过很遗憾没有中文的详细资料
asr(金币+3,VIP+0)::P 非常感谢，欢迎常来交流！辛苦了
hshzhq(金币+1,VIP+0):谢谢了，不过很遗憾没有中文的详细资料
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Masson’s trichrome染色，主要用于区别胶原纤维与其他组织，常用于如肝硬化等实验动物模式。 原理、方法等见附件 Principle Trichrome procedures are so named because three dyes, which may or may not include the nuclear stain, are used. Sections are first stained with an acid dye such as Biebrich scarlet. All acidophilic tissue elements such as cytoplasm, muscle and collagen will bind with the acid dyes. The sections are then treated with phosphotungstic and/or phosphomolybdic acid. Because cytoplasm is much less permeable than collagen the phosphotungstic and phosphomolybdic acids cause the Biebrich scarlet to diffuse out of the collagen but not out of the cytoplasm. Phosphotungstic and phosphomolybdic acids have numerous acidic groups that most likely act as a link between the decolorized collagen and aniline blue, the collagen dye. Probably the pH of the phosphotungstic/phosphomolybdic acid solution also increases selective collagen staining and aids in the diffusion or removal of Biebrich scarlet. [ Last edited by dajiahaoma on 2008-3-13 at 08:33 ] |
2楼2008-03-13 08:26:05
