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[½»Á÷] ÓÐË­ÖªµÀMasson¡®s trichrome stain¼´massonÈýɫȾɫ¡¢ÂíÊÏÈýɫȾɫ·¨µÄÔ­ÀíºÍÒâÒå¡£

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[ Last edited by hshzhq on 2008-3-12 at 22:26 ]
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dajiahaoma

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Masson¡¯s trichromeȾɫ£¬Ö÷ÒªÓÃÓÚÇø±ð½ºÔ­ÏËάÓëÆäËû×éÖ¯£¬³£ÓÃÓÚÈç¸ÎÓ²»¯µÈʵÑ鶯Îïģʽ¡£
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Principle
Trichrome procedures are so named because three dyes, which may or may not include the
nuclear stain, are used. Sections are first stained with an acid dye such as Biebrich scarlet.
All acidophilic tissue elements such as cytoplasm, muscle and collagen will bind with the
acid dyes. The sections are then treated with phosphotungstic and/or phosphomolybdic acid.
Because cytoplasm is much less permeable than collagen the phosphotungstic and
phosphomolybdic acids cause the Biebrich scarlet to diffuse out of the collagen but not out of
the cytoplasm. Phosphotungstic and phosphomolybdic acids have numerous acidic groups
that most likely act as a link between the decolorized collagen and aniline blue, the collagen
dye. Probably the pH of the phosphotungstic/phosphomolybdic acid solution also increases
selective collagen staining and aids in the diffusion or removal of Biebrich scarlet.

[ Last edited by dajiahaoma on 2008-3-13 at 08:33 ]
2Â¥2008-03-13 08:26:05
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