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clcelvis金虫 (小有名气)
小木虫
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[求助]
看到一篇文献，无奈知识贮备有限，还请大神指教其中荧光测试原理。
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看到一篇文献，无奈知识贮备有限，还请大神指教。那篇文献是Magnetic Iron Oxide Nanoworms for Tumor Targeting and Imaging** ByJi-Ho Park, Geoffrey von Maltzahn, Lianglin Zhang, Michael P. Schwartz, Erkki Ruoslahti, Sangeeta N. Bhatia,andMichael J. Sailor* Figure 2. Internalization of nanoworms (NWs) and nanospheres (NSs) conjugated with F3 peptides into MDA-MB-435 cells. Conceptual scheme illustrating the increased multivalent interactions expected between receptors on a cell surface and targeting ligands on a NW compared with a NS. b) Fluorescence data comparing the efficiency of cellular internalization for various functionalized NW and NS systems. NH2, F3, and PEG-F3 indicate aminated NW/NS, F3-conjugated NW/NS and PEGylgated F3-conjugated NW/NS, respectively. c) Fluorescence microscope images of cells 3 h after incubation with F3(FITC)-conjugated NW (NW-175-F) or F3(FITC)-conjugated NS (NS-30-F) (green). Nuclei are visualized with a DAPI stain (blue). Scale bar is 20mm 我不理解荧光测试的测试机理？为什么缩氨酸分子与细胞结合就能显示荧光？不结合就不能显示荧光？ |
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