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| Characterization and growth optimization of a novel salt resistance Pseudomonas putida strain Zm-1 capable of degrading trichloroethylene by cometabolism under aerobic conditions[J]. Asian Journal of Chemistry. 2013, 25(15): 8718-8722 |
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fukkim: ½ð±Ò+1, ±»Ë÷ÒýÁËÂ𣿠2014-03-24 11:22:11
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Characterization and Growth Optimization of a Novel Salt Resistant Pseudomonas putida Strain Zm Capable of Degrading Trichloroethylene by Cometabolism Under Aerobic Conditions ×÷Õß:Zhang, X (Zhang, X.)[ 1 ] ; Xu, D (Xu, D.)[ 2 ] ASIAN JOURNAL OF CHEMISTRY ¾í: 25 ÆÚ: 15 Ò³: 8718-8722 ×Ó¼: A ³ö°æÄê: NOV 2013 ²é¿´ÆÚ¿¯ÐÅÏ¢ ÕªÒª A novel toluene-oxidizing strain capable of cometabolizing trichloroethylene was isolated and characterized. Combined with 16S rRNA sequence analysis, it was identified as Pseudomonas sp. It could grow with toluene as the sole carbon source. The analysis of the growth curve showed that Zm could reach the logarithmic phase within 8-10 h. Continuous sampling showed that toluene was exhausted within 1 d. Finally 29.1 % of trichloroethylene (50 mg/L) can be removed after incubation in mineral salt medium in the presence of 200 mg/L toluene for 3 d and 62.7 % for 7 d. The optimization of growth conditions was also conducted, the optimal growth conditions as the follows: carbon source toluene, at the concentration of 200-400 mg/L, medium pH 7, temperature 28 degrees C and shaking speed 200 rpm. The tested strain Zm also showed substantial salt resistance including at 3.5 % NaCl concentration, indicating the potential application in bioremediation of coastal and marine trichloroethylene contaminants. ¹Ø¼ü´Ê ×÷Õ߹ؼü´Ê:Aerobic cometabolism; Biodegradation; Pseudomonas putida; Trichloroethylene; Salt tolerance KeyWords Plus EGRADATION; BIODEGRADATION; TOLUENE; TCE; F1×÷ÕßÐÅÏ¢ ͨѶ×÷ÕßµØÖ·: Zhang, X (ͨѶ×÷Õß) Yancheng Teachers Univ, Jiangsu Prov Key Lab Coastal Wetland Bioresources, Yancheng 224051, Peoples R China. µØÖ·: [ 1 ] Yancheng Teachers Univ, Jiangsu Prov Key Lab Coastal Wetland Bioresources, Yancheng 224051, Peoples R China [ 2 ] Yancheng Teachers Univ, Coll Life Sci & Technol, Yancheng 224051, Peoples R China µç×ÓÓʼþµØÖ·:yongshengzh@163.com »ù½ð×ÊÖúÖÂл »ù½ð×ÊÖú»ú¹¹ ÊÚȨºÅ National Natural Science Foundation of China 10905035 Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection Jiangsu Key Laboratory for Bioresources of Saline Soils ²é¿´»ù½ð×ÊÖúÐÅÏ¢ ³ö°æÉÌ ASIAN JOURNAL OF CHEMISTRY, 11/100 RAJENDRA NAGAR, SECTOR 3,, SAHIBABAD 201 005, GHAZIABAD, INDIA Àà±ð / ·ÖÀà Ñо¿·½Ïò:Chemistry Web of Science Àà±ð:Chemistry, Multidisciplinary ÎÄÏ×ÐÅÏ¢ ÎÄÏ×ÀàÐÍ:Article ÓïÖÖ:English Èë²ØºÅ: WOS:000325204200110 ISSN: 0970-7077 ÆÚ¿¯ÐÅÏ¢ Impact Factor (Ó°ÏìÒò×Ó): Journal Citation Reports® ÆäËûÐÅÏ¢ IDS ºÅ: 228RF Web of Science ºËÐĺϼ¯ÖÐµÄ "ÒýÓõIJο¼ÎÄÏ×": 18 Web of Science ºËÐĺϼ¯ÖÐµÄ "±»ÒýƵ´Î": 0 |
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