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wulv008银虫 (正式写手)
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[求助]
跪求大神支招，Genbank这么说怎么解释
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[2] We have noticed that some or all of the sequences in file "2222-C.sqn" contain reading frame shifts (insertions/ deletions based on BLAST similarity search results and/or an alignment, see attached): Insertion at alignment position 14 Gap: 32 Non-gap: 2 C-HZ029,C-HZ010 Insertion at alignment position 54 Gap: 33 Non-gap: 1 C-HZ029 Deletion at alignment position 554 Gap: 1 Non-gap: 33 C-HZ032 Insertion at alignment position 563 Gap: 33 Non-gap: 1 C-HZ010 Insertion at alignment position 564 Gap: 33 Non-gap: 1 C-HZ010 Insertion at alignment position 569 Gap: 33 Non-gap: 1 C-HZ033 This generally indicates problems such as: - errors in the nucleotide sequence - sequences have not been trimmed of low quality - problems with feature annotation which may be due to: translation in the wrong reading frame or on the wrong strand translation does not have the correct exon spans translation includes noncoding sequence, such as introns or UTR's If the sequences and/or annotation are incorrect, please resubmit with the corrected files. If the information is correct, please explain the presence of the internal stop codons and/or frame shifts. If you are unable to determine the correct sequence and/or annotation for your sequence(s), they can still be processed and the following comment will be added to your records: COMMENT GenBank staff is unable to verify sequence and/or annotation provided by the submitter. In addition, the record(s) will indicate that the data are unverified and your sequence(s) will not be included in NCBI BLAST databases. Please either: [1] resubmit with correct sequence(s) and/or annotation or [2] confirm that your sequence(s) should be processed as unverified. 不想重新测序，我应该怎么解释，感谢各位专家老师帮我分析一下，该怎么解决这种问题，万分感谢！ |
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