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wulv008

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[求助] 跪求大神支招，Genbank这么说怎么解释

[2] We have noticed that some or all of the sequences in file
"2222-C.sqn" contain reading frame shifts (insertions/
deletions based on BLAST similarity search results and/or an
alignment, see attached):

Insertion at alignment position 14
Gap: 32 Non-gap: 2
C-HZ029,C-HZ010

Insertion at alignment position 54
Gap: 33 Non-gap: 1
C-HZ029

Deletion at alignment position 554
Gap: 1 Non-gap: 33
C-HZ032

Insertion at alignment position 563
Gap: 33 Non-gap: 1
C-HZ010

Insertion at alignment position 564
Gap: 33 Non-gap: 1
C-HZ010

Insertion at alignment position 569
Gap: 33 Non-gap: 1
C-HZ033

This generally indicates problems such as:

  - errors in the nucleotide sequence
  - sequences have not been trimmed of low quality
  - problems with feature annotation which may be due to:
translation in the wrong reading frame or on the wrong strand
translation does not have the correct exon spans
translation includes noncoding sequence, such as introns or UTR's

If the sequences and/or annotation are incorrect, please resubmit
with the corrected files. If the information is correct, please explain
the presence of the internal stop codons and/or frame shifts.

If you are unable to determine the correct sequence and/or annotation
for your sequence(s), they can still be processed and the following
comment will be added to your records:

COMMENT  GenBank staff is unable to verify sequence and/or annotation
      provided by the submitter.

In addition, the record(s) will indicate that the data are unverified and
your sequence(s) will not be included in NCBI BLAST databases.

Please either:

[1] resubmit with correct sequence(s) and/or annotation
or
[2] confirm that your sequence(s) should be processed as unverified.

不想重新测序，我应该怎么解释，感谢各位专家老师帮我分析一下，该怎么解决这种问题，万分感谢！

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