| 查看: 1472 | 回复: 0 | ||
wulv008银虫 (正式写手)
|
[求助]
跪求大神支招，Genbank这么说怎么解释
|
|
[2] We have noticed that some or all of the sequences in file "2222-C.sqn" contain reading frame shifts (insertions/ deletions based on BLAST similarity search results and/or an alignment, see attached): Insertion at alignment position 14 Gap: 32 Non-gap: 2 C-HZ029,C-HZ010 Insertion at alignment position 54 Gap: 33 Non-gap: 1 C-HZ029 Deletion at alignment position 554 Gap: 1 Non-gap: 33 C-HZ032 Insertion at alignment position 563 Gap: 33 Non-gap: 1 C-HZ010 Insertion at alignment position 564 Gap: 33 Non-gap: 1 C-HZ010 Insertion at alignment position 569 Gap: 33 Non-gap: 1 C-HZ033 This generally indicates problems such as: - errors in the nucleotide sequence - sequences have not been trimmed of low quality - problems with feature annotation which may be due to: translation in the wrong reading frame or on the wrong strand translation does not have the correct exon spans translation includes noncoding sequence, such as introns or UTR's If the sequences and/or annotation are incorrect, please resubmit with the corrected files. If the information is correct, please explain the presence of the internal stop codons and/or frame shifts. If you are unable to determine the correct sequence and/or annotation for your sequence(s), they can still be processed and the following comment will be added to your records: COMMENT GenBank staff is unable to verify sequence and/or annotation provided by the submitter. In addition, the record(s) will indicate that the data are unverified and your sequence(s) will not be included in NCBI BLAST databases. Please either: [1] resubmit with correct sequence(s) and/or annotation or [2] confirm that your sequence(s) should be processed as unverified. 不想重新测序，我应该怎么解释，感谢各位专家老师帮我分析一下，该怎么解决这种问题，万分感谢！ |
回复此楼» 猜你喜欢
留学--博士招生
已经有16人回复
-
化学工程，本211，求调剂，ab区不限
已经有4人回复
-
化学工程及工业化学论文润色/翻译怎么收费?
已经有212人回复
-
湖北师范大学招调剂啦
已经有7人回复
-
邵阳学院食品与化学工程学院硕士调剂
已经有0人回复
-
天津商业大学 生物与医药课题组招生
已经有0人回复
-
生物化工学硕招收调剂
已经有0人回复
-
广东石油化工学院2026年硕士研究生需要多名生物，制药工程，食品专业的调剂生
已经有0人回复
-
317分 一志愿江南大学 化学工程学硕 求调剂
已经有6人回复
-
化工申博
已经有1人回复
找到一些相关的精华帖子，希望有用哦~
跪求大神支招，Genbank这么说怎么解释
已经有10人回复
- 向genbank发送sequin邮件要写怎么写？大家平安夜快乐呦~~
已经有4人回复
- 我向Genbank提交了序列并收到了登录号，可我把那封邮件弄没了，怎么再获得登录号？
已经有12人回复
- 已拿到Genbank的accession number，想修改作者信息，怎么办？
已经有3人回复
点击这里搜索更多相关资源科研从小木虫开始，人人为我，我为人人
