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【答案】应助回帖
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秋水晚风: 金币+20, 翻译EPI+1, ★★★★★最佳答案 2014-03-13 18:14:30
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We firstly used the PORTRAIT software to predict the non-coding RNA of two target pests transcripts ,then the predicted results was compared with the RNA database NONCODE, RFAM, RNA DB for homology to identify the non-coding RNA in insect transcriptome .Multiple non-coding RNA was found in S. furcifera and few in other species .It is believed that there is no conserved protein-coding gene on non-coding RNA gene sequence and homology analysis based on sequence conservation can not be effective in finding non-coding RNA.Another reason is that solexa sequencing strategy may not be conducive to the enrichment of non-coding RNA gene sequence and lots of non-coding RNA gene information losted .In other words ,transcriptome may not be valid data for non-coding RNA analysis . |
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