| 查看: 523 | 回复: 2 | |||
| 本帖产生 1 个 翻译EPI ，点击这里进行查看 | |||
ygy19901025木虫 (著名写手)
|
[求助]
生物类摘要，求翻译英译汉，金币奉上
|
||
|
Abstract Glycogen synthase kinase-3β (GSK-3β), a key regulator of neuronal apoptosis, is inhibited by the phosphorylation of Ser-9/Ser-389 and was recently shown to be cleaved by calpain at the N terminus, leading to its subsequent activation. In this study calpain was found to cleave GSK-3β not only at the N terminus but also at the C terminus, and cleavage sites were identified at residues Thr-38–Thr-39 and Ile-384–Gln-385. Furthermore, the cleavage of GSK-3β occurred in tandem with Ser-9 dephosphorylation during cerebellar granule neuron apoptosis. Increasing Ser-9 phosphorylation of GSK-3β by inhibiting phosphatase 1/2A or pretreating with purified active Akt inhibited calpain-mediated cleavage of GSK-3β at both N and C termini, whereas non-phosphorylatable mutant GSK-3β S9A facilitated its cleavage. In contrast, Ser-389 phosphorylation selectively inhibited the cleavage of GSK-3β at the C terminus but not the N terminus. Calpain-mediated cleavage resulted in three truncated products, all of which contained an intact kinase domain: ΔN-GSK-3β (amino acids 39–420), ΔC-GSK-3β (amino acids 1–384), and ΔN/ΔC-GSK-3β (amino acids 39–384). All three truncated products showed increased kinase and pro-apoptotic activity, with ΔN/ΔC-GSK-3β being the most active form. This observation suggests that the GSK-3β C terminus acts as an autoinhibitory domain similar to the N terminus. Taken together, these findings demonstrate that calpain-mediated cleavage activates GSK-3β by removing its N- and C-terminal autoinhibitory domains and that Ser-9 phosphorylation inhibits the cleavage of GSK-3β at both termini. In contrast, Ser-389 phosphorylation inhibits only C-terminal cleavage but not N-terminal cleavage. These findings also identify a mechanism by which site-specific phosphorylation and calpain-mediated cleavage operate in concert to regulate GSK-3β activity. |
回复此楼» 猜你喜欢
职称评审没过，求安慰
已经有10人回复
-
EST投稿状态问题
已经有4人回复
-
聘U V热熔胶研究人员
已经有10人回复
-
求助文献
已经有3人回复
-
垃圾破二本职称评审标准
已经有10人回复
-
投稿返修后收到这样的回复，还有希望吗
已经有8人回复
-
三无产品还有机会吗
已经有6人回复
-
谈谈两天一夜的“延安行”
已经有13人回复
-
氨基封端PDMS和HDI反应快速固化
已经有11人回复
ygy19901025
木虫 (著名写手)
- 应助: 24 (小学生)
- 金币: 1298.3
- 散金: 1690
- 红花: 9
- 沙发: 7
- 帖子: 2956
- 在线: 106.7小时
- 虫号: 2614551
- 注册: 2013-08-24
- 专业: 神经生物学
wangyuan0929
金虫 (小有名气)
- 翻译EPI: 12
- 应助: 10 (幼儿园)
- 金币: 706.2
- 红花: 8
- 帖子: 88
- 在线: 21.6小时
- 虫号: 2233248
- 注册: 2013-01-10
- 专业: 环境微生物学
【答案】应助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ...
ygy19901025: 金币+100, 翻译EPI+1, ★★★★★最佳答案 2014-03-13 22:11:24
ygy19901025: 金币+100, 翻译EPI+1, ★★★★★最佳答案 2014-03-13 22:11:24