| 查看: 10215 | 回复: 338 | ||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | ||
[资源]
Elsevier 2014 新书：纯化及鉴定实验手册
|
||
|
书名 Experiments in the Purification and Characterization of Enzymes --A Laboratory Manual 作者 Thomas Crowley / Jack Kyte 出版商 Elsevier -Academic Press 出版日期 Feb 27, 2014 页数 266 书号 ISBN: 978-0-12-409544-1 书评 Written in a logical, easy-to-understand manner, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual is an indispensable resource for both students and instructors in the fields of biochemistry, molecular biology, chemistry, pharmaceutical chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics. 目录 Safety Guidelines for Biochemistry Laboratories General Guidelines for Handling Solutions of Protein Maintaining a Laboratory Notebook Introduction to Enzymes Catalyzing Oxidation-Reductions with the Coenzyme NAD(P) Computational Techniques for Biochemistry Software for Analysis of Data Computational Methods and Bioinformatics for Studying the Structure and Function of Proteins Section 1: FNR; Purification and Characterization of Ferredoxin-NADP+ Reductase from Chloroplasts of S. oleracea 1. Preparation of a Lysate of Chloroplasts and Sequestration of Proteins by an Anion-exchange Solid Phase; 2. Elution of Proteins from the Anion-exchange Solid Phase and Assays for the Concentration of Total Protein and FNR Activity; 3. Isolation of FNR by Adsorption and Elution 4. Electrophoresis of Proteins from the Chloroplasts and Purified FNR on Gels of Polyacrylamide Cast in Solutions of Dodecyl Sulfate; 5. Kinetic Assay for the Km of FNR for NADPH; 6. Estimation of the Size and Quaternary Structure of Native FNR Using Chromatography by Molecular Exclusion; 7. Detection of FNR in Extracts from Chloroplasts of Spinach by Immunoblotting Section 2: LuxG; Purification and Characterization of a Recombinant FMN Reductase from P. leiognathi 1. Expression of LuxG-hexahistidine in E. coli and Its Release from the Cells by Lysis; 2. Affinity Adsorption of LuxG-hexahistidine to a Solid Phase to which Ni2+ is Chelated; 3. Electrophoresis of the Polypeptides in the Lysate and Purified LuxG-hexahistidine; 4. Assay for the Flavin Reductase Activity of LuxG-hexahistidine; 5. Determination of the Michaelis Constant of LuxG-hexahistidine for NADH; 6. Determination of the Molar Concentration of LuxG-hexahistidine Spectrophotometrically Section 3: LDH; Purification and Characterization of Bovine LDH 1. Preparation of the Isoenzymes of LDH from Extracts of Various Tissues; 2. Spectrophotometric Assay of the Activity of Bovine LDH; 3. Separation and Visualization of the Isoenzymes of LDH by Electrophoresis on Cellulose Acetate; 4. Fractionation of Proteins in Bovine Heart by Precipitation with Sulfate; 5. Purification of LDH from Heart by Affinity Adsorption and Elution; 6. Concentration of Protein by the Bradford Stain and Absorbance at 280 nm; 7. Electrophoresis of Native Proteins from Bovine Heart and Purified LDH on a Gel of Polyacrylamide; 8. Electrophoresis of Unfolded Polypeptides from Bovine Heart and Purified LDH Saturated with Dodecyl Sulfate; 9. Determination of the Michaelis Constant, KmNADH, of the Isoenzymes of LDH for NADH; 10. Chromatography by Molecular Exclusion to Evaluate the Quaternary Structure of LDH; 11. Detection of LDH in Extracts of Bovine Tissue by Immunoblotting Section 4: Experimental Design; 1. Association of FNR with the Membranes of the Thylakoids in the Chloroplasts from Spinach; 2. Search for Multiple Flavin Reductases in P. leiognathi; 3. Assay for LDH Activity Specific to (R)-lactate in Bovine Mitochondria Appendices I. Measurement of Absorbance with Multiwell Plates and an Automated Plate Reader; II. Operation of the Spectronic 20D+ Spectrophotometer 封面截图 -  Experiments in the Purification and Characterization of Enzymes-pic.png |
回复此楼» 本帖附件资源列表
-
欢迎监督和反馈：小木虫仅提供交流平台，不对该内容负责。
本内容由用户自主发布，如果其内容涉及到知识产权问题，其责任在于用户本人，如对版权有异议，请联系邮箱：xiaomuchong@tal.com - 附件 1 : Experiments_in_the_Purification_and_Characterization_of_Enzymes.rar
2014-03-12 01:18:16, 8.09 M
» 本帖已获得的红花（最新10朵）
代帶待» 猜你喜欢
推荐一些20种氨基酸检测的实际应用案例
已经有0人回复
-
不合理蛙科研实验中的趣事：实验器材的 “乌龙”
已经有0人回复
-
化学工程及工业化学论文润色/翻译怎么收费?
已经有146人回复
-
不合理蛙科研实验中的趣事：和实验材料的 “斗智斗勇”
已经有0人回复
-
蛋白质检测：精准分析，解锁生物分子的密码
已经有0人回复
-
不合理蛙科研实验之小鼠实验：严谨设计，解析生命机制的重要载体
已经有0人回复
-
不合理蛙科研实验之重金属检测：精准筛查，守护健康与环境的防线
已经有0人回复
-
不合理蛙科研实验之“蛙测重金属我背锅三千”
已经有0人回复
-
不合理蛙科研实验之“鼠逃三次我发三篇SCI”
已经有0人回复
-
纳米粒度分析
已经有1人回复
64楼2014-03-16 13:39:31
简单回复
jinhx872楼
2014-03-12 07:16
回复
五星好评 顶一下，感谢分享！
2014-03-12 08:29
回复
五星好评 顶一下，感谢分享！
2014-03-12 13:17
回复
五星好评 顶一下，感谢分享！
qhuzhl5楼
2014-03-12 14:49
回复
五星好评 顶一下，感谢分享！
2014-03-12 14:58
回复
五星好评 顶一下，感谢分享！
yuhengc8楼
2014-03-13 06:46
回复
五星好评 顶一下，感谢分享！
2014-03-13 08:39
回复
五星好评 顶一下，感谢分享！
lieying1810楼
2014-03-13 08:51
回复
五星好评 顶一下，感谢分享！
muxinjin11楼
2014-03-13 09:02
回复
五星好评 顶一下，感谢分享！
xl007133412楼
2014-03-13 09:16
回复
五星好评 顶一下，感谢分享！
2014-03-13 10:22
回复
五星好评 顶一下，感谢分享！
雨夜的风14楼
2014-03-13 10:37
回复
五星好评 顶一下，感谢分享！
