²é¿´: 3319  |  »Ø¸´: 2

js2008wjw

Í­³æ (³õÈëÎÄ̳)

[ÇóÖú] DH5a¸ÐÊÜ̬ϸ°ûÀ©ÔöÖÊÁ£×öÀ¶°×°ßɸѡ±ØÐëÒª¼ÓIPTGÓÕµ¼Ã´£¿ ÒÑÓÐ1È˲ÎÓë

×î½üÔÚѧϰDH5a¸ÐÊÜ̬ϸ°ûµÄÄÚÈÝ£¬¿´µ½Ò»¸ö×ÊÁÏDH5aȱÉÙLacIq»ùÒò£¬Òò´ËÔÚ×öÀ¶°×°ßɸѡµÄʱºò²»ÐèҪʹÓÃIPTG£¬Ö»¼ÓX-gal¾Í¿ÉÒÔÁË£»¶øJM109¸ÐÊÜ̬ÓÐLacIq£¬ÐèÒªIPTG+X-galͬʱ´æÔÚ²ÅÄÜÓÕµ¼³öÀ¶°×°ß¡£ÓÉÓÚûÓÐʵÑéÌõ¼þÑéÖ¤£¬ËùÒÔÇëÎÊÓÐûÓÐÈË×ö¹ýDH5a²»¼ÓIPTGÒ²ÄÜÓÕµ¼³öÀ¶°×°ßµÄʵÑ飿
»Ø¸´´ËÂ¥

» ²ÂÄãϲ»¶

» ±¾Ö÷ÌâÏà¹Ø¼ÛÖµÌùÍÆ¼ö£¬¶ÔÄúͬÑùÓаïÖú:

ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

ÌìʹÍÐ

ר¼Ò¹ËÎÊ (Ö°Òµ×÷¼Ò)

T.Shen

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¡ï
¸Ðл²ÎÓ룬ӦÖúÖ¸Êý +1
dllchina: ½ð±Ò+1, ˵µÄû´í£¬ÊµÑéÔ­ÀíÊÇÖ¸µ¼·½Õë°¡ 2014-03-06 00:27:10
Ó¡ÏóÖ®ÖкÃÏñ²»ÓüÓIPTGÓÕµ¼²»£¿IPTGÔÚÓÕµ¼µ°°×µÄʱºòÒª¼Ó...
ÄãÒªÁ˽âÀ¶°×°ßµÄÔ­Àí¾Í»áÇå³þΪºÎ²»¼ÓIPTGÁË...
ÏȸãÇå³þÔ­Àí¡£
Whenever,Wherever,Anyway!MustRemember:Fight!KeepFighting!NeverGiveUp!
2Â¥2014-03-05 23:18:33
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

js2008wjw

Í­³æ (³õÈëÎÄ̳)

ÒýÓûØÌû:
2Â¥: Originally posted by ÌìʹÍÐ at 2014-03-05 23:18:33
Ó¡ÏóÖ®ÖкÃÏñ²»ÓüÓIPTGÓÕµ¼²»£¿IPTGÔÚÓÕµ¼µ°°×µÄʱºòÒª¼Ó...
ÄãÒªÁ˽âÀ¶°×°ßµÄÔ­Àí¾Í»áÇå³þΪºÎ²»¼ÓIPTGÁË...
ÏȸãÇå³þÔ­Àí¡£

Ô­ÀíÎÒÊǸãÇå³þÁË£¬ÏÖÔÚÊÇÏëÎÊÏÂÓÐûÓÐÈË×ö¹ýÕâ¸öʵÑéÑéÖ¤
3Â¥2014-03-06 09:22:26
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
Ïà¹Ø°æ¿éÌø×ª ÎÒÒª¶©ÔÄÂ¥Ö÷ js2008wjw µÄÖ÷Ìâ¸üÐÂ
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] 292Çóµ÷¼Á +7 ¶ì¶ì¶ì¶î¶î¶î¶î¶ 2026-03-25 8/400 2026-03-26 00:22 by Dyhoer
[¿¼ÑÐ] 289Çóµ÷¼Á +14 ˶ÐǸ° 2026-03-23 14/700 2026-03-25 22:22 by 544594351
[¿¼ÑÐ] 321Çóµ÷¼Á +3 è±Óñ~~ 2026-03-25 3/150 2026-03-25 19:07 by Zhanglab-TJU
[¿¼ÑÐ] ÇóbÇøÔºÐ£µ÷¼Á +4 ÖÜ56 2026-03-24 5/250 2026-03-25 17:12 by yishunmin
[¿¼ÑÐ] 282Çóµ÷¼Á +3 wcq131415 2026-03-24 3/150 2026-03-25 12:16 by userper
[¿¼ÑÐ] 0854µç×ÓÐÅÏ¢Çóµ÷¼Á 324 +4 Promise-jyl 2026-03-23 4/200 2026-03-25 11:36 by Sugarlight
[¿¼ÑÐ] 278Çóµ÷¼Á +4 ÎÒ¿ÉÒÔÉϰ¶µÄ¶Ô 2026-03-19 4/200 2026-03-25 11:01 by userper
[¿¼ÑÐ] 287Çóµ÷¼Á +10 ³¿»èÏßÓëÐǺ£ 2026-03-19 11/550 2026-03-25 10:35 by userper
[¿¼ÑÐ] »¯Ñ§µ÷¼Á +6 yzysaa 2026-03-21 6/300 2026-03-25 09:27 by aa331100
[¿¼ÑÐ] Ò»Ö¾Ô¸ÎäÀí085500»úеרҵ×Ü·Ö300Çóµ÷¼Á +3 an10101 2026-03-24 7/350 2026-03-25 00:00 by ɽ¹í0-
[¿¼ÑÐ] ʳƷר˶ һ־Ը˫һÁ÷ 328 +3 xiaom99 2026-03-21 4/200 2026-03-24 21:20 by lailaisimei
[¿¼ÑÐ] ²ÄÁÏ¿¼Ñе÷¼ÁÉú +3 »ÆÁ»Ò»ÃÎǧÄê 2026-03-24 3/150 2026-03-24 17:00 by barlinike
[¿¼ÑÐ] 277·ÖÇóµ÷¼Á£¬¿çµ÷²ÄÁÏ +3 ¿¼Ñе÷¼Álxh 2026-03-24 3/150 2026-03-24 13:52 by JourneyLucky
[¿¼ÑÐ] Ò»Ö¾Ô¸±±¾©»¯¹¤´óѧ070300 ѧ˶336Çóµ÷¼Á +5 vvÃÔ 2026-03-21 8/400 2026-03-22 14:20 by ColorlessPI
[¿¼ÑÐ] Çóµ÷¼Á +5 Zhangbod 2026-03-21 7/350 2026-03-22 13:13 by Zhangbod
[¿¼ÑÐ] 303Çóµ÷¼Á +5 °²ÒäÁé 2026-03-22 6/300 2026-03-22 12:46 by ËØÑÕÇã³Ç1988
[¿¼ÑÐ] 22 350 ±¾¿Æ985Çóµ÷¼Á£¬ÇóÀϵÇÊÕÁô +3 ÀîéóÄÐ003 2026-03-20 3/150 2026-03-21 13:28 by ²«»÷518
[¿¼ÑÐ] Ò»Ö¾Ô¸Î÷ÄϽ»Í¨ ר˶ ²ÄÁÏ355 ±¾¿ÆË«·Ç Çóµ÷¼Á +5 Î÷ÄϽ»Í¨×¨²Ä355 2026-03-19 5/250 2026-03-20 21:10 by JourneyLucky
[¿¼ÑÐ] 353Çóµ÷¼Á +3 À­¹³²»Ðí±ä 2026-03-20 3/150 2026-03-20 19:56 by JourneyLucky
[¿¼ÑÐ] ²ÄÁÏ¿¼Ñе÷¼Á +3 xwt¡£ 2026-03-19 3/150 2026-03-19 11:22 by wãåÑôw
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û