| 查看: 1871 | 回复: 4 | |||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | |||
[交流]
求助酶活测定方法 PAL、CHS、F3H、DFR、LDOX
|
|||
|
谁能提供以下植物中 酶活性测定方法： PAL、CHS、F3H、DFR、LDOX 谢谢了！ |
回复此楼» 猜你喜欢
欢迎发来filecode的Mz6后的代码验证其规律
已经有70人回复
-
有时候，自然基金真的不能太认真 （我的申报经验）
已经有12人回复
-
招聘 金属平磨液，抛光液研发工程师
已经有3人回复
-
时间戳又变了8-15
已经有23人回复
-
filecode=后面第一个是大写字母
已经有7人回复
-
filecode
已经有12人回复
-
小木虫上这么多卖论文的，真有人买论文么？感觉没必要啊
已经有13人回复
-
哪位老哥知道今年的国自然具体哪一天放榜？
已经有7人回复
-
关于Filecode分析方法
已经有13人回复
-
各位道友，我要去昆明玩几天，回来见。
已经有8人回复
» 抢金币啦！回帖就可以得到:
-
2026年财务管理、经济与信息技术国际会议 (FMEIT 2026)
+1/673
-
西安交大马伟/王亚洲课题组诚聘联培博士和硕士学生多名-有机生物电子学方向
+1/185
-
2026年生物学、大数据与信息工程国际会议 (BSBIE 2026)
+1/95
-
北京信息科技大学机电工程学院硕士研究生招生线上宣讲会——8月16日
+1/81
-
中国科学院空天院精密干涉测量团队招聘光学/算法/机械方向博士后/特别研究助理/工程师
+1/79
-
上海一恒贝茵公司全国均可联系销售或售后服务
+1/69
-
中国科大-合肥国家实验室冷原子量子网络团队招聘启事
+2/56
-
清华大学深圳国际研究生院招聘-博士后（长期有效）
+1/29
-
华南理工大学招2027年生物材料、药物递送申请考核制博士生
+1/29
-
祈福！
+1/27
-
祈福
+1/21
-
西北工业大学 生科院 秦绪军课题组诚招博士后、博士/硕士研究生【长期有效】
+1/13
-
中山大学国家级青年人才课题组招收推免硕士2名（2027年入学）
+2/4
-
中国科学院生态环境研究中心环境工程相关方向招收保送硕士和联合培养博士和硕士研究生
+1/4
-
中国科学院生物物理研究所田勇课题组招聘联合培养生
+1/3
-
海南大学化学院—功能分子器件团队2027博士招生+博士后招聘
+1/2
-
KFUPM Turki Baroud组招全奖博士/博后｜下一代膜材料・水凝胶・清洁水
+1/2
-
中国地质大学（武汉）3D打印微纳光刻方向招收申请考核博士生
+1/2
-
悉尼科技大学（QS 第87）数据科学/AI招收2027年秋季入学全奖及CSC博士生
+1/1
-
中国科学技术大学苏州高等研究院张振教授2026级硕士生招生（推免）
+1/1
4楼2017-10-18 21:48:59
2楼2014-09-15 14:32:02
★
小木虫: 金币+0.5, 给个红包，谢谢回帖
小木虫: 金币+0.5, 给个红包，谢谢回帖
|
这个好像很难，我查到了2种方法 第一种：ASSAY OF CHALCONE SYNTHASE ACTIVITY BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY，PhytochemistVryo,l . 34,N o. 5, pp. 1225-1229，1993 第二种： CHS[12] chaleone synthase，查儿酮合成酶 Chemicals, [2-14C]Malonyl-CoA (1.71GBq•mmo1-1) was purchased from N.E.N. (Boston, Mass., USA) and [U-14C]phenyl-alanine (16.65GBq•mmol-1) from Amersham (Braunschweig, Germany). 4-Coumaroyl-CoA was synthesized by Dr. P.A. Bäumker (Institut für Botanik, Münster, Germany; Bäumker et al. 1988)[13]. Enzyme preparations. ö Pbenylalanine ammonia-lyase: Leaves were cut into small pieces, frozen at -20°C and homogenized in a prechilled mortar in cold acetone (- 20°C The homogenate was filtered and the residue washed several times with cold acetone. The resulting dry powder was suspended in 0.1 M sodium borate buffer (pH 8.8) containing 5 mM dithioerythritol (DTE). Chalcone synthase. All steps were carried out at 0-4°C Leaf material was homogenized in 0.1 M potassium phosphate buffer (pH 6.8) containing 40 mM ascorbate, 1 mM EDTA, 10 mM thiourea硫脲, 5 mM DTE and 0.25 g Polyclar AT.(g FW)-1 (Serva, Heidelberg, Germany). The homogenate was centrifuged for 10 min at 10 000 g and the supernatant concentrated by ultrafiltration (Diaflo PM 30; Amicon, Witten, Germany). Enzyme assays, The activity of PAL was measured by the method of Amrhein and Zenk (1971). The activity of CHS was determined according to Beerhues and Wiermann (1988)[14], except that the pH of the incubation buffer was 7.8 and the final DTE concentration 0.5 mM. The analysis of the reaction products was performed as described by Lembach et al. (1989)[15]. (CHS activity was determined according to Beerhues and Wiermann (1985)[16]. The analysis of the reaction product was carried out using thin layer chromatography with silica gel plates and hexane: ethyl-acetate: methanol = 50:50:l (v/v/v) as a solvent system. For re-chromatography of the eluted product cellulose plates and 15% (v/v) ethanol were employed.) Protein determination. Protein concentration was measured according to the method of Bradford (1976), as modified by Read and Northcote (1981), using ovalbumin as a standard. Schöpker, H., M. Kneisel, L. Beerhues, et al. Phenylalanine ammonia-lyase and chalcone synthase in glands of Primula kewensis (W. Wats): immunofluorescence and immunogold localization. Planta, 1995. 196(4): 712-719. 13. PA, B., A. S and W. R. Metabolism of ferulic acid sucrose esters in anthers of Tulipa cv. Apeldoorn. II. Highly specific degradation of the esters by different esterase activities. Z. Naturforsch, 1988. 43c: 647-655. |
3楼2014-12-26 14:49:21
5楼2018-08-05 13:29:58
