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[交流] 爱丁堡FLS980和HORIBA FL-3荧光光谱仪哪个好？

实验室想购买荧光光谱仪，需要满足以下要求：
1. 稳态光谱测试
2. 绝对荧光量子效率测试
3. 瞬态光谱测试，即荧光寿命测试
4. 光谱范围：紫外可见近红外

目前调研的仪器有爱丁堡FLS980和HORIBA FL-3可满足要求，不知道有没有同学用过这两款仪器，那个更好一些？或者这两款仪器都有哪些问题？

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1楼 2014-02-18 09:26:25

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Fluorolog @ Nature
23篇论文可以作为证据说明FL-3的性价比更好--投入产出比

1. Hajin Kim, Sanjaya C. Abeysirigunawarden, Ke Chen, Megan Mayerle, Kaushik Ragunathan, Protein-guided RNA dynamics during early ribosome assembly, Nature 506, 334-338 doi:10.1038/nature13039  12 February 2014

2. Yoni Haitin, Anne E. Carlson & William N. Zagotta, The structural mechanism of KCNH-channel regulation by the eag domain, Nature 501, 444-448 doi:10.1038/nature12487  25 August 2013

3. Julian Burschka, Norman Pellet, Soo-Jin Moon, Robin Humphry-Baker, Peng Gao, Sequential deposition as a route to high-performance perovskite-sensitized solar cells, Nature 499, 316-319 doi:10.1038/nature12340 10 July 2013

4. Yong Ju Kim, Klaus Peter Hofmann, Oliver P. Ernst, Patrick Scheerer, Hui-Woog Choe, Crystal structure of pre-activated arrestin p44, Nature 497, 142-146 doi:10.1038/nature12133 21 April 2013

5. Hui-Woog Choe, Yong Ju Kim, Jung Hee Park, Takefumi Morizumi, Emil F. Pai, Crystal structure of metarhodopsin, Nature 471, 651-655 doi:10.1038/nature09789  9 March 2011

6. Cuimin Liu, Anna L. Young, Amanda Starling-Windhof, Andreas Bracher, Sandra Saschenbrecker, Coupled chaperone action in folding and assembly of hexadecameric Rubisco, Nature 463, 197-202 doi:10.1038/nature08651 14 January 2010

7. Ivy E. Dick, Michael R. Tadross, Haoya Liang, Lai Hock Tay, Wanjun Yang , A modular switch for spatial Ca2+ selectivity in the calmodulin regulation of CaV channels, Nature 451, 830-834 doi:10.1038/nature06529  30 January 2008

8. Petr Kaláb, Arnd Pralle, Ehud Y. Isacoff, Rebecca Heald & Karsten Weis, Analysis of a RanGTP-regulated gradient in mitotic somatic cells, Nature 440, 697-701 doi:10.1038/nature04589 30 March 2006

9. Andrew A. Pascal, Zhenfeng Liu, Koen Broess, Bart van Oort, Herbert van Amerongen, Molecular basis of photoprotection and control of photosynthetic light-harvesting, Nature 436, 134-137 doi:10.1038/nature03795 7 July 2005

10. Steven C. Erwin, Lijun Zu, Michael I. Haftel, Alexander L. Efros, Thomas A. Kennedy, Doping semiconductor nanocrystals, Nature 436, 91-94 doi:10.1038/nature03832  7 July 2005

11. Seok-Yong Lee & Roderick MacKinnon, A membrane-access mechanism of ion channel inhibition by voltage sensor toxins from spider venom, Nature 430, 232-235 doi:10.1038/nature02632  8 July 2004

12. A. V. Ruban, M. Wentworth, A. E. Yakushevska, J. Andersson, P. J. Lee, Plants lacking the main light-harvesting complex retain photosystem II macro-organization, Nature 421, 648-652 doi:10.1038/nature01344, 6 February 2003

13. Benjamin Schuler, Everett A. Lipman & William A. Eaton, Probing the free-energy surface for protein folding with single-molecule fluorescence spectroscopy, Nature 419, 743-747 doi:10.1038/nature01060, 17 October 2002

14. J. Kim & T. M. Swager, Control of conformational and interpolymer effects in conjugated polymers, Nature 411, 1030-1034 doi:10.1038/35082528,  28 June 2001

15. Hardware harvest  Nature 401, 509-512 doi:10.1038/46843  30 September 1999

16. Klaus van Leyen, Robert M. Duvoisin, Harald Engelhardt & Martin Wiedmann, A function for lipoxygenase in programmed organelle degradation, Nature 395, 392-395 doi:10.1038/26500, 24 September 1998

17. Andrea Montali, Cees Bastiaansen, Paul Smith & Christoph Weder, Polarizing energy transfer in photoluminescent materials for display applications, Nature 392, 261-264 doi:10.1038/32616, 19 March 1998

18. Earl Danielson, Josh H. Golden, Eric W. McFarland, Casper M. Reaves, W. Henry Weinberg, A combinatorial approach to the discovery and optimization of luminescent materials, Nature 389, 944-948 doi:10.1038/40099 30 October 1997

19. Ferdinand Rossi, Emmanuel Labourier, Thierry Forné, Gilles Divita, Jean Derancourt, Specific phosphorylation of SR proteins by mammalian DNA topoisomerase I, Nature 381, 80-82 doi:10.1038/381080a0 2 May 1996

20. Thomas Langer, Chi Lu, Harrison Echols, John Flanagan, Manajit K. Hayer, Successive action of DnaK, DnaJ and GroEL along the pathway of chaperone-mediated protein folding, Nature 356, 683-689 doi:10.1038/356683a0

21. Jörg Martin, Thomas Langer, Raina Boteva, Andrea Schramel, Arthur L. Horwich, Chaperonin-mediated protein folding at the surface of groEL through a 'molten globule'-like intermediate, Nature 352, 36-42 doi:10.1038/352036a0  4 July 1991

22. David I. Ratner, Equivalence of intracellular pH of differentiating Dictyostelium cell types, Nature 321, 180-182 doi:10.1038/321180a0 8 May 1986

23. M. Nakache, A. B. Schreiber, H. Gaub & H. M. McConnell, Heterogeneity of membrane phospholipid mobility in endothelial cells depends on cell substrate, Nature 317, 75-77 doi:10.1038/317075a0    5 September 1985