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Transcriptome of Vector, and the Molecular Interactions of virus Transmission
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Transcriptome of the Plant Virus Vector Graminella nigrifrons, and the Molecular Interactions of Maize fine streak virus Transmission Abstract Background: Leafhoppers (Hemiptera: Cicadellidae) are plant-phloem feeders that are known for their ability to vector plant pathogens. The black-faced leafhopper (Graminella nigrifrons) has been identified as the only known vector for the Maize fine streak virus (MFSV), an emerging plant pathogen in the Rhabdoviridae. Within G. nigrifrons populations, individuals can be experimentally separated into three classes based on their capacity for viral transmission: transmitters, acquirers and non-acquirers. Understanding the molecular interactions between vector and virus can reveal important insights in virus immune defense and vector transmission. Results: RNA sequencing (RNA-Seq) was performed to characterize the transcriptome of G. nigrifrons. A total of 38,240 ESTs of a minimum 100 bp were generated from two separate cDNA libraries consisting of virus transmitters and acquirers. More than 60% of known D. melanogaster, A. gambiae, T. castaneum immune response genes mapped to our G. nigrifrons EST database. Real time quantitative PCR (RT-qPCR) showed significant down-regulation of three genes for peptidoglycan recognition proteins (PGRP – SB1, SD, and LC) in G. nigrifrons transmitters versus control leafhoppers. Conclusions: Our study is the first to characterize the transcriptome of a leafhopper vector species. Significant sequence similarity in immune defense genes existed between G. nigrifrons and other well characterized insects. The down-regulation of PGRPs in MFSV transmitters suggested a possible role in rhabdovirus transmission. The results provide a framework for future studies aimed at elucidating the molecular mechanisms of plant virus vector competence. |
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zmfzj: 金币+55, 翻译EPI+1 2014-02-10 07:26:35
zmfzj: 金币+55, 翻译EPI+1 2014-02-10 07:26:35
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黑面（脸）叶蝉植物病毒载体转录（物）组及其在玉米细条纹病毒传播时与玉米细条纹病毒分子的相互作用 摘要 背景：叶蝉（半翅目：叶蝉科）被称为植物韧皮部馈线，因其能携带植物病原体而得名。黑脸叶蝉（Graminella nigrifrons）已被确定为（炮弹病毒）弹状病毒科唯一已知的玉米细条纹病毒（MFSV）载体，一个新兴的植物病原体。黑脸叶蝉种群个体可通过实验基于他们对病毒传播能力分为三类：传染源，acquirers and non-acquirers。了解病毒载体和病毒分子间的相互作用可以洞察病毒免疫防御和病毒载体传播的重要关键。 结果：RNA测序（RNA的序号）表征黑脸叶蝉转录组。总共产生38,240个长度超过100 BP的基因表达序列标签（Expressed Sequence Tag ，EST ），可从两个不同的包含病毒传染源和 acquirers的cDNA库比对。已知的黑腹果蝇，冈比亚按蚊，赤拟谷盗免疫反应基因 ，60％以上可以映射到黑脸叶蝉基因表达序列标签数据库。实时定量PCR（RT-qPCR的）显示黑脸叶蝉与对照叶蝉相比，三个与肽聚糖识别蛋白（PGRP - SB1，SD，和LC）有关的基因显著下调。 结论：本研究第一次表征叶蝉载体物种的转录组。在黑脸叶蝉与其他已进行很好表征的昆虫之间免疫防御基因存在显著序列相似 。MFSV传染源PGRPs下调提示可能是弹状病毒传播的作用。研究结果对未来旨在阐明植物病毒载体能力分子机制的研究提供了一个框架。 这是一篇2012PLOS ONE 上的文章，acquirers ， non-acquirers在病毒学上是指什么东西不太清楚。 |
2楼2014-02-09 23:52:55
