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Briefly, lyophilized purified peptide was dissolved in a minimum amount of 2 mM NaOH and
the pH is adjusted to 10.5 using 100 mM NaOH. The peptide solution is sonicated for 2 minutes,
and then filtered through 0.2 ¦Ìm centrifuge filters. The filtered solution is adjusted to 400 ¦ÌL
using sodium phosphate buffer (100 mM sodium phosphate at pH 7.4, 600 mM NaCl)



The fabrication of the A¦Â fibril is so simple that
requires only incubation of monomeric A¦Â at 37 C in phosphate
buffer (pH 7.4) over sufficient time (1 h under seed-mediated
condition).
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