24小时热门版块排行榜    

查看: 1243  |  回复: 1

yetaieva

铁杆木虫 (著名写手)

[求助] 关于贝塔淀粉样蛋白纤维化

在文献中看到两种纤维化的方式,请问这两种方式得到的产物fibril 是一致的么?

Briefly, lyophilized purified peptide was dissolved in a minimum amount of 2 mM NaOH and
the pH is adjusted to 10.5 using 100 mM NaOH. The peptide solution is sonicated for 2 minutes,
and then filtered through 0.2 μm centrifuge filters. The filtered solution is adjusted to 400 μL
using sodium phosphate buffer (100 mM sodium phosphate at pH 7.4, 600 mM NaCl)



The fabrication of the Aβ fibril is so simple that
requires only incubation of monomeric Aβ at 37 C in phosphate
buffer (pH 7.4) over sufficient time (1 h under seed-mediated
condition).
回复此楼
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

小雷雷yl

新虫 (初入文坛)

请问楼主用这两种方法制备出来了吗

发自小木虫Android客户端
2楼2019-09-24 06:47:37
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
相关版块跳转 我要订阅楼主 yetaieva 的主题更新
信息提示
请填处理意见