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ÔÚÎÄÏ×Öп´µ½Á½ÖÖÏËά»¯µÄ·½Ê½£¬ÇëÎÊÕâÁ½ÖÖ·½Ê½µÃµ½µÄ²úÎïfibril ÊÇÒ»ÖµÄô£¿ Briefly, lyophilized purified peptide was dissolved in a minimum amount of 2 mM NaOH and the pH is adjusted to 10.5 using 100 mM NaOH. The peptide solution is sonicated for 2 minutes, and then filtered through 0.2 ¦Ìm centrifuge filters. The filtered solution is adjusted to 400 ¦ÌL using sodium phosphate buffer (100 mM sodium phosphate at pH 7.4, 600 mM NaCl) The fabrication of the A¦Â fibril is so simple that requires only incubation of monomeric A¦Â at 37 C in phosphate buffer (pH 7.4) over sufficient time (1 h under seed-mediated condition). |
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- ½ð±Ò: 12.8
- Ìû×Ó: 19
- ÔÚÏß: 5Сʱ
- ³æºÅ: 15106459
- ×¢²á: 2019-05-15
- ÐÔ±ð: MM
- רҵ: ʳƷ¿ÆÑ§»ù´¡
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