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liyan20220: 金币+5, 翻译EPI+1 2014-01-14 20:30:45

This paper  mainly focus on the following  three parts:
1 Preparation and molecular mechanisms of  ORBK antimicrobial peptides .  Realized the ORBK antimicrobial peptide fusion protein MBP's fusion expression and purification using genetic engineering method and  obtained cyclic ORBK antimicrobial peptides  through  separation of  the mixed linear and cyclic ORBK antimicrobial peptides by HPLC after digestion method and H2O2 oxidation .
2The mechanism of  most of antimicrobial peptides is  destroy the structure of cell membranes which caused permeation of  a large number of  cell contents and resulted  in bacterial death.  We used  liposome system and  Nanodiscs and to study the mechanism of ORBK  antimicrobial peptides  . Result  showed  the ORBK antimicrobial peptides's effect on bacterial membrane  caused the  leakage of the contents and resulted  in bacterial death.
3. D29P63 and PK34 are D29 Mycobacteriophage  peptides which can be used to prepare  anti-TB drugs formulations. We successfully obtained  these two peptides by  genetic engineering method  and  for  the first  time completely  identified their main chain atoms  by  liquid nuclear magnetic resonance technology

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