| 查看: 1159 | 回复: 0 | ||
[求助]
求助翻译，关于LIVE/DEAD assay这个实验方法的 急用~~~
|
|
篇文献用到LIVE/DEAD assay这个实验方法，其中的步骤有一段不太理解，求大神帮助！！ Cells were detached from the substrate with Trypsin and counted prior to centrifugation. Prior to hydrogel crosslinking, 25,000 cells were suspended in 100 lL aliquots of each hydrogel precursor solution (n ¼ 3). Each aliquot was transferred to wells in a 96-well plate after which the solutions were allowed to crosslink or crosslinking was initiated by the appropriate method. After gelation, 100 lL of Keratinocyte SFM was added to each well. After 24 h in culture, media was removed and 100 lL of 4 mM calcein-AM and 4 mM ethidium homodimer-1 assay solution was added to each well. |
回复此楼» 猜你喜欢
5个%2F，非固定段22个字母
已经有7人回复
-
伯胺上甲基求助
已经有5人回复
-
投英文期刊如何查重
已经有3人回复
-
生命口C13面上会评时间
已经有10人回复
-
生命口面上会评结束了吗大家有知道结果吗？现在还没有结果正常不？
已经有7人回复
-
生生命学部会评
已经有4人回复
-
H口，面上，时间戳7月7日。
已经有3人回复
-
小木虫没落了，除了祈祷帖子，几乎看不到有价值的帖子
已经有14人回复
-
会评
已经有5人回复
-
E口会评
已经有13人回复