| 查看: 1045 | 回复: 0 | ||
[求助]
求助翻译，关于LIVE/DEAD assay这个实验方法的 急用~~~
|
|
篇文献用到LIVE/DEAD assay这个实验方法，其中的步骤有一段不太理解，求大神帮助！！ Cells were detached from the substrate with Trypsin and counted prior to centrifugation. Prior to hydrogel crosslinking, 25,000 cells were suspended in 100 lL aliquots of each hydrogel precursor solution (n ¼ 3). Each aliquot was transferred to wells in a 96-well plate after which the solutions were allowed to crosslink or crosslinking was initiated by the appropriate method. After gelation, 100 lL of Keratinocyte SFM was added to each well. After 24 h in culture, media was removed and 100 lL of 4 mM calcein-AM and 4 mM ethidium homodimer-1 assay solution was added to each well. |
回复此楼» 猜你喜欢
职称评审没过，求安慰
已经有41人回复
-
回收溶剂求助
已经有7人回复
-
硝基苯如何除去
已经有3人回复
-
A期刊撤稿
已经有4人回复
-
垃圾破二本职称评审标准
已经有17人回复
-
投稿Elsevier的Neoplasia杂志，到最后选publishing options时页面空白，不能完成投稿
已经有22人回复
-
EST投稿状态问题
已经有7人回复
-
毕业后当辅导员了，天天各种学生超烦
已经有4人回复
-
求助文献
已经有3人回复
-
三无产品还有机会吗
已经有6人回复