| 查看: 1190 | 回复: 0 | ||
[求助]
求助翻译，关于LIVE/DEAD assay这个实验方法的 急用~~~
|
|
篇文献用到LIVE/DEAD assay这个实验方法，其中的步骤有一段不太理解，求大神帮助！！ Cells were detached from the substrate with Trypsin and counted prior to centrifugation. Prior to hydrogel crosslinking, 25,000 cells were suspended in 100 lL aliquots of each hydrogel precursor solution (n ¼ 3). Each aliquot was transferred to wells in a 96-well plate after which the solutions were allowed to crosslink or crosslinking was initiated by the appropriate method. After gelation, 100 lL of Keratinocyte SFM was added to each well. After 24 h in culture, media was removed and 100 lL of 4 mM calcein-AM and 4 mM ethidium homodimer-1 assay solution was added to each well. |
回复此楼» 猜你喜欢
申博发邮件
已经有8人回复
-
各位大神，目前国内有哪些比较好用的逆合成软件？
已经有9人回复
-
国社科系统bug了，是不是要放榜了？
已经有7人回复
-
上海工程技术大学激光智能制造课题组｜2027级博士研究生招生公告
已经有8人回复
-
上海工程技术大学激光智能制造课题组招收博士研究生
已经有8人回复