| 查看: 1197 | 回复: 0 | ||
[求助]
求助翻译，关于LIVE/DEAD assay这个实验方法的 急用~~~
|
|
篇文献用到LIVE/DEAD assay这个实验方法，其中的步骤有一段不太理解，求大神帮助！！ Cells were detached from the substrate with Trypsin and counted prior to centrifugation. Prior to hydrogel crosslinking, 25,000 cells were suspended in 100 lL aliquots of each hydrogel precursor solution (n ¼ 3). Each aliquot was transferred to wells in a 96-well plate after which the solutions were allowed to crosslink or crosslinking was initiated by the appropriate method. After gelation, 100 lL of Keratinocyte SFM was added to each well. After 24 h in culture, media was removed and 100 lL of 4 mM calcein-AM and 4 mM ethidium homodimer-1 assay solution was added to each well. |
回复此楼» 猜你喜欢
我应该选哪条合成路线让实验成功率高一些？
已经有8人回复
-
烦躁
已经有8人回复
-
入职后第一次管课题组的专利缴费，对账才发现官费比别人多掏了一倍
已经有3人回复
-
各位大佬，路线设计求助
已经有7人回复