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Acrylate-modified nucleoside (dNBA) was dried at 80 ¡ãC for 2 h in vacuo. After cooling, PO(OMe)3 and POCl3 were added on ice under argon atmosphere.
The resulting mixture was stirred at 0 ¡ãC. The phosphorylation was stopped by addition of TEAB (2 M, 2 mL) and water (2 mL). The
products were purified by C18 reversed-phase HPLC using water/methanol (5 to 100%) containing 0.1 M TEAB buffer as eluent.
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2Â¥: Originally posted by xiaochun85 at 2013-12-31 16:18:11
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