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真强必胜: 金币+1, thanks 2013-12-18 21:45:04
liyan20220: 金币+5, 翻译EPI+1 2013-12-18 21:59:43
We also tried using Tricine-SDS-PAGE to detect the occurrence of  the proteolytic cleavage .   Due to the  greater  MBP fusion protein and the very small  target protein , we can see  target protein with huge sample volume and can not determine whether the protein is completely digested . So we reduced the sample size to determine whether the proteolytic cleavage  is complete.
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