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| We show that nonmuscle MIIB (myosin-IIB) is unpolarized in cells on soft matrix in 2D and also within soft 3D collagen, with rearward polarization of MIIB emerging only as cells migrate from soft to stiff matrix. Durotaxis is the tendency of cells to crawl from soft to stiff matrix, and durotaxis of primary mesenchymal stem cells (MSCs) proved more sensitive to MIIB than to the more abundant and persistently unpolarized nonmuscle MIIA¡£ |
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ÕæÇ¿±ØÊ¤: ½ð±Ò+1 2013-12-05 13:57:45
ÕæÇ¿±ØÊ¤: ½ð±Ò+1 2013-12-05 13:57:45
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ÎÒÃÇ·¢ÏÖ£¬ÔÚ2DºÍ3D½ºÔµ°°×Èí»ùÖÊÖеÄϸ°ûÉϵķǼ¡ÈâMIIB£¨¼¡Çòµ°°×-IIB£©ÊǷǼ«ÐԵ쬽öµ±Ï¸°û´ÓÈíµ½Ó²»ùÖÊÇ¨ÒÆÊ±·¢ÉúMIIB¼«»¯¡£ DurotaxisÊÇϸ°û´ÓÈíµ½Ó²»ùÖÊÐнøµÄÇãÏò£¬³õ¼¶¼ä³äÖʸÉϸ°û£¨MSCs£©µÄdurotaxisÖ¤Ã÷¶ÔMIIB±È¸ü·á¸»¡¢¸üÄÑÒÔ¼«»¯µÄ·Ç¼¡ÈâMIIA¸üÃô¸Ð¡£ durotaxis£ºÇ÷Ó²ÐÔ¡£duroÀ¶¡ÎÄÓ²µÄ£¬taxisÊÇÉúÎïµÄÇ÷ÐÔ£¬½áºÏÏà¹ØÎÄÏ×---cell prefers to migrate towards stiffer regions on a substrate with a stiffness gradient£¬the phenomenon is called durotaxis |

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