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[资源] 2014新书尝鲜，Biosensors: Essentials

Lecture Notes In Chemistry 84， Springer，2014
Biosensors:Essentials
Gennady Evtugyn
Department of Analytical Chemistry
Kazan Federal University
Kazan
Russia

Contents

1 Introduction and Overview of History . . . 1
1.1 Short Biosensor History . . . 4
1.2 Biosensor Classification . . .7
References . . .18

2 Biochemical Components Used in Biosensor Assemblies . . . 21
2.1 Enzymes. . .22
2.2 Antibodies . . . 42
2.3 Protein/Peptide Receptors . . .51
2.4 Nucleic Acids . . .53
2.5 Whole Cells as Biosensing Elements . . . 62
2.6 Immobilization of Biochemical Elements of Biosensors . . .65
2.6.1 General Requirements for Immobilization Techniques . . . 65
2.6.2 Physical Immobilization. . . 71
2.6.3 Covalent Immobilization . . . 84
References . . .91

3 Biosensor Signal Transducers. . . 99
3.1 Electrochemical Transducers . . .99
3.1.1 Potentiometric Sensors. . . 101
3.1.2 Voltammetric Sensors . . .115
3.1.3 Conductometric and Impedimetric Devices . . .157
3.2 Optical Transducers . . . 168
3.2.1 Optosensors . . .168
3.2.2 Bioluminescence and Electrogenerated Luminescence Devices. . .174
3.2.3 Surface Plasmon Resonance and SERS . . .180
3.3 Other Techniques . . . 185
3.3.1 Quartz Crystal Microbalances. . .185
3.3.2 Calorimetric Biosensors . . .190
3.3.3 MEMS Systems . . . 194
References . . .198

4 How Does It Work? Case Studies . . .207
4.1 The Biggest Success: Glucose Meters . . .208
4.2 From Chemical Weapons Toward Pesticides: Anticholinesterase Sensing . . .212
4.3 Immunosensors Versus Test Strips: Hapten Determination . . .221
4.4 DNA Chips: The Human Genome Project and More . . .226
4.5 Toxicity Bioassay: Microtox Related Systems. . . 230
References . . . 238

5 Biosensor Prospects: Quo Vadis? (Conclusion) . . .243
References . . . 250

Appendix A: Enzyme Kinetics . . .251
Appendix B: Inhibition Kinetics. . .255

Index . . . 261

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