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1. Little expression difference of Let-7 family members, however£¬was observed
between BCSCs and MCF-7 cells£¬with the exception of Let-7e.
£¨×¢£ºBCSCΪbreast cancer stem cellµÄËõд£©
2. These results suggested that DNMT3A was not responsible for DNA methylation in the promoter£¬first exon or first intron regulatory regions of Oct4£®
3. Cantz et al. considered that HeLa cells did not express Oct4£¬but the other two reports confirmed that Oct4 was expressed in HeLa cells.
4. Besides our study, Nehls et al. also confirmed that in patients with cervical cancer£¬p16 was strongly expressed despite hypermethylation in exon la of p16.
5. The specificity analysis demonstrated that hybridization did not occur between
nucleotide sequences with a single-base mismatch£¬which is partly consistent with what
Thomas Naiser and E. Lorenzo has reported£¨Garcia£¬et al.£¬2008£»Naiser£¬et al.£¬2008)£®

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