24小时热门版块排行榜

>论坛更新日志 (5032)
>考研 (2198)
>导师招生 (1710)
>虫友互识 (445)
>文献求助 (325)
>硕博家园 (177)
>考博 (176)
>休闲灌水 (145)
>博后之家 (135)
>招聘信息布告栏 (81)
>论文投稿 (65)
>基金申请 (54)
>教师之家 (43)
>标准与专利 (35)
>找工作 (33)
>论文道贺祈福 (30)

返回列表

当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖

流风易逝

铁杆木虫 (正式写手)

应助: 0 (幼儿园)
金币: 7997
帖子: 347
在线: 250.3小时
虫号: 1446441

[交流] 【2013-12-31】Frontiers in Microbiology专刊征稿【SCIE】

截稿日期:

2013年12月31日

DNA Replication Origins in Microbial Genomes

导师主持的专刊，如有合适的工作，欢迎各位投稿。如有兴趣，可站内信联系。

http://www.frontiersin.org/evolu ... origins_in_mic/2193

In collaboration with Frontiers in Microbiology, we are organizing a Research Topic titled "DNA Replication Origins in Microbial Genomes”.

Frontiers, a Swiss open-access publisher, recently partnered with Nature Publishing Group to expand its researcher-driven Open Science platform. Frontiers articles are rigorously peer-reviewed, can be disseminated freely and are widely read by your colleagues and by the broader scientific and medical research communities.

Research Topic
DNA replication, a central event for cell proliferation, is the basis of biological inheritance. Complete and accurate DNA replication is integral to the maintenance of the genetic integrity of organisms. In all three domains of life, DNA replication begins at replication origins. In bacteria, replication initiates from a single replication origin (oriC), which contains several DnaA boxes. In eukaryotic genomes, replication initiates from significantly more replication origins, ranging from hundreds in yeast to tens of thousands in human, activated simultaneously at a specific time. For eukaryotic organisms, replication origins are best characterized in the unicellular eukaryote budding yeast Saccharomyces cerevisiae and the fission yeast Schizosaccharomyces pombe. The budding yeast origins contain an essential sequence element called the ARS (autonomously replicating sequence), while the fission yeast origins consist of AT-rich sequences. Within the archaeal domain, the multiple replication origins have been identified by a predict-and-verify approach in the hyperthermophilic archaeon Sulfolobus. Archaeal origins contain two or more short repetitive DNA sequences, known as origin recognition boxes (ORBs). It appears that archaea have a simplified version of the eukaryotic replication apparatus, which has led to considerable interest in the archaeal machinery as a model of that in eukaryotes.

The identification of replication origins is important not only in providing insights into the structure and function of the replication origins but also in understanding the regulatory mechanisms of the initiation step in DNA replication. Therefore, intensive studies have been carried out on the identification of replication origins in the last two decades. The pioneer work to identify bacterial oriCs in silico is the GC-skew analysis. Later, a method of cumulative GC skew without sliding windows was proposed to give better resolution. Meanwhile, an oligomer-skew method was also proposed to predict oriC regions in bacterial genomes. As a unique representation of a DNA sequence, the Z-curve method has been proved to be an accurate and effective approach to predict bacterial and archaeal replication origins. Budding yeast origins have been predicted by Oriscan using similarity to the characterized ones, while the fission yeast origins have been indentified initially from AT content calculation. In comparison with the in silico analysis, the experimental methods are convincing and reliable, but time-consuming and labor-intensive. The microbial replication origins can be identified by several experimental methods including construction of replicative oriC plasmids, microarray-based or high-throughput sequencing-based marker frequency analysis, two-dimensional gel electrophoresis analysis and replication initiation point mapping (RIP mapping) etc. The recent genome-wide approaches to identify and characterize replication origin locations have boosted the number of mapped yeast replication origins. The availability of increasing complete microbial genomes and emerging approaches has created challenges and opportunities for identification of their replication origins in silico, as well as in vivo.

This research topic invites for the studies of microbial replication origins, addressing all the issues mentioned above, by in silico analyses as well as in vivo experiments. Manuscripts describing original research, methods, opinions, reviews are all welcome, which would provide a comprehensive overview of this field.

[ Last edited by 流风易逝 on 2013-11-4 at 12:03 ]

回复此楼

» 猜你喜欢

070300化学学硕311分求调剂已经有17人回复
本科211 工科085400 280分求调剂可跨专业已经有10人回复
342电子信息专硕求调剂已经有9人回复
080500求调剂已经有15人回复
人工智能320调剂08工类还有机会吗已经有10人回复
326求调剂已经有4人回复
还有化工二轮调剂的学校吗已经有44人回复
290求调剂已经有5人回复
生物学308求调剂（一志愿华东师大）已经有5人回复
考研调剂已经有26人回复

» 抢金币啦！回帖就可以得到:

查看全部散金贴

1楼 2013-11-04 11:50:29

已阅关注TA 给TA发消息送TA红花 TA的回帖

szydz

新虫 (小有名气)

应助: 0 (幼儿园)
金币: 419.2
帖子: 55
在线: 18小时
虫号: 126091

顶顶顶顶顶顶顶顶顶顶

赞一下

回复此楼

65楼2014-04-25 16:01:58